Genetic and antigenic characterization of the Amur virus associated with hemorrhagic fever with renal syndrome

Kumari Lokugamage, Hiroaki Kariwa, Nandadeva Lokugamage, Hironobu Miyamoto, Masahiro Iwasa, Tomohiro Hagiya, Koichi Araki, Atsushi Tachi, Tetsuya Mizutani, Kumiko Yoshimatsu, Jiro Arikawa, Ikuo Takashima

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The genetic and antigenic characteristics of the Amur (AMR) and Far East (FE) virus lineages, which are both within the genus Hantavirus, were studied. Representative viruses, H5 and B78 for AMR and Bao 14 for FE, were used. The entire small (S) and medium (M) segments, except for the 3′- and 5′-ends, were sequenced. The deduced amino acid sequences of AMR had 96.7 and 92.0-92.2% identities with the Hantaan (HTN) virus in the S and M segments, respectively. The amino acid sequences of FE had 99.1 and 97.9% identities in the S and M segments, respectively. The three viral strains and HTN virus had similar binding patterns to a panel of monoclonal antibodies (MAbs), except that one MAb did not bind AMR. However, sera from Apodemus peninsulae, naturally infected with AMR virus, neutralized homologous viruses at 1:160 to 1:320 dilutions and HTN at 1:20 to 1:40 dilutions. The anti-AMR serum neutralized homologous viruses at a 1:80 dilution and HTN at a 1:40 dilution. The anti-HTN serum did not neutralize AMR (

Original languageEnglish (US)
Pages (from-to)127-134
Number of pages8
JournalVirus Research
Volume101
Issue number2
DOIs
StatePublished - May 2004
Externally publishedYes

Fingerprint

Hemorrhagic Fever with Renal Syndrome
Far East
Viruses
Hantaan virus
Amino Acid Sequence
Serum
Hantavirus
Murinae
Monoclonal Antibodies

Keywords

  • Amur
  • Bunyaviridae
  • Hantaan
  • Hantavirus
  • Hantavirus infections
  • Hemorrhagic fever with renal syndrome

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

Cite this

Lokugamage, K., Kariwa, H., Lokugamage, N., Miyamoto, H., Iwasa, M., Hagiya, T., ... Takashima, I. (2004). Genetic and antigenic characterization of the Amur virus associated with hemorrhagic fever with renal syndrome. Virus Research, 101(2), 127-134. https://doi.org/10.1016/j.virusres.2003.12.031

Genetic and antigenic characterization of the Amur virus associated with hemorrhagic fever with renal syndrome. / Lokugamage, Kumari; Kariwa, Hiroaki; Lokugamage, Nandadeva; Miyamoto, Hironobu; Iwasa, Masahiro; Hagiya, Tomohiro; Araki, Koichi; Tachi, Atsushi; Mizutani, Tetsuya; Yoshimatsu, Kumiko; Arikawa, Jiro; Takashima, Ikuo.

In: Virus Research, Vol. 101, No. 2, 05.2004, p. 127-134.

Research output: Contribution to journalArticle

Lokugamage, K, Kariwa, H, Lokugamage, N, Miyamoto, H, Iwasa, M, Hagiya, T, Araki, K, Tachi, A, Mizutani, T, Yoshimatsu, K, Arikawa, J & Takashima, I 2004, 'Genetic and antigenic characterization of the Amur virus associated with hemorrhagic fever with renal syndrome', Virus Research, vol. 101, no. 2, pp. 127-134. https://doi.org/10.1016/j.virusres.2003.12.031
Lokugamage, Kumari ; Kariwa, Hiroaki ; Lokugamage, Nandadeva ; Miyamoto, Hironobu ; Iwasa, Masahiro ; Hagiya, Tomohiro ; Araki, Koichi ; Tachi, Atsushi ; Mizutani, Tetsuya ; Yoshimatsu, Kumiko ; Arikawa, Jiro ; Takashima, Ikuo. / Genetic and antigenic characterization of the Amur virus associated with hemorrhagic fever with renal syndrome. In: Virus Research. 2004 ; Vol. 101, No. 2. pp. 127-134.
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