Genetic and antigenic diversity among eastern equine encephalitis viruses from North, Central, and South America

Aaron C. Brault, Ann M. Powers, Cesar Luis Villarreal Chavez, Roberto Navarro Lopez, Moises Fraire Cachón, Luis Fernando Liera Gutierrez, Wenli Kang, Robert B. Tesh, Robert E. Shope, Scott Weaver

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92 Citations (Scopus)

Abstract

Eastern equine encephalitis virus (EEEV), the sole species in the EEE antigenic complex, is divided into North and South American antigenic varieties based on hemagglutination inhibition tests. Here we describe serologic and phylogenetic analyses of representatives of these varieties, spanning the entire temporal and geographic range available. Nucleotide sequencing and phylogenetic analyses revealed additional genetic diversity within the South American variety; 3 major South/Central American lineages were identified including one represented by a single isolate from eastern Brazil, and 2 lineages with more widespread distributions in Central and South America. All North American isolates comprised a single, highly conserved lineage with strains grouped by the time of isolation and to some extent by location. An EEEV strain isolated during a 1996 equine outbreak in Tamaulipas State, Mexico was closely related to recent Texas isolates, suggesting southward EEEV transportation beyond the presumed enzootic range. Plaque reduction neutralization tests with representatives from the 4 major lineages indicated that each represents a distinct antigenic subtype. A taxonomic revision of the EEE complex is proposed.

Original languageEnglish (US)
Pages (from-to)579-586
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume61
Issue number4
StatePublished - Oct 1999

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Eastern equine encephalitis virus
Central America
Antigenic Variation
South America
North America
Hemagglutination Inhibition Tests
Neutralization Tests
Mexico
Horses
Disease Outbreaks
Brazil
Nucleotides

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Brault, A. C., Powers, A. M., Villarreal Chavez, C. L., Navarro Lopez, R., Cachón, M. F., Gutierrez, L. F. L., ... Weaver, S. (1999). Genetic and antigenic diversity among eastern equine encephalitis viruses from North, Central, and South America. American Journal of Tropical Medicine and Hygiene, 61(4), 579-586.

Genetic and antigenic diversity among eastern equine encephalitis viruses from North, Central, and South America. / Brault, Aaron C.; Powers, Ann M.; Villarreal Chavez, Cesar Luis; Navarro Lopez, Roberto; Cachón, Moises Fraire; Gutierrez, Luis Fernando Liera; Kang, Wenli; Tesh, Robert B.; Shope, Robert E.; Weaver, Scott.

In: American Journal of Tropical Medicine and Hygiene, Vol. 61, No. 4, 10.1999, p. 579-586.

Research output: Contribution to journalArticle

Brault, AC, Powers, AM, Villarreal Chavez, CL, Navarro Lopez, R, Cachón, MF, Gutierrez, LFL, Kang, W, Tesh, RB, Shope, RE & Weaver, S 1999, 'Genetic and antigenic diversity among eastern equine encephalitis viruses from North, Central, and South America', American Journal of Tropical Medicine and Hygiene, vol. 61, no. 4, pp. 579-586.
Brault AC, Powers AM, Villarreal Chavez CL, Navarro Lopez R, Cachón MF, Gutierrez LFL et al. Genetic and antigenic diversity among eastern equine encephalitis viruses from North, Central, and South America. American Journal of Tropical Medicine and Hygiene. 1999 Oct;61(4):579-586.
Brault, Aaron C. ; Powers, Ann M. ; Villarreal Chavez, Cesar Luis ; Navarro Lopez, Roberto ; Cachón, Moises Fraire ; Gutierrez, Luis Fernando Liera ; Kang, Wenli ; Tesh, Robert B. ; Shope, Robert E. ; Weaver, Scott. / Genetic and antigenic diversity among eastern equine encephalitis viruses from North, Central, and South America. In: American Journal of Tropical Medicine and Hygiene. 1999 ; Vol. 61, No. 4. pp. 579-586.
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