Genetic and antigenic diversity of Ehrlichia chaffeensis

Comparative analysis of a novel human strain from Oklahoma and previously isolated strains

Sheng Min Chen, Xue Jie Yu, Vsevolod Popov, Eric L. Westerman, Frank G. Hamilton, David Walker

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

A new Ehrlichia strain, designated as Ehrlichia chaffeensis, Sapulpa strain, was isolated from a patient from Oklahoma with severe ehrlichiosis. Isolation of the ehrlichial pathogen was achieved by inoculating patient blood onto HEL cells and DH82 cells. Antigenic properties of the new isolate were characterized with monoclonal antibodies, homologous patient serum, and polyclonal rabbit serum by Western immunoblotting. The results showed antigenic differences and protein size variation of Sapulpa strain compared with the other 2 strains of E. chaffeensis. Sequencing of the 16S rRNA gent showed 100% identity to that of E. chaffeensis, strain 91HE17. Polymerase chain reaction and sequencing of DNA homologous to the 120-kDa protein gent of E. chaffeensis, Arkansas strain, showed that this gene of Sapulpa strain was smaller than that of Arkansas strain and contained a repeat region with three tandem repeat units.

Original languageEnglish (US)
Pages (from-to)856-863
Number of pages8
JournalJournal of Infectious Diseases
Volume175
Issue number4
StatePublished - 1997

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Ehrlichia chaffeensis
Antigenic Variation
Ehrlichia
Ehrlichiosis
Tandem Repeat Sequences
Serum
DNA Sequence Analysis
Proteins
Western Blotting
Monoclonal Antibodies
Rabbits
Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Genetic and antigenic diversity of Ehrlichia chaffeensis : Comparative analysis of a novel human strain from Oklahoma and previously isolated strains. / Chen, Sheng Min; Yu, Xue Jie; Popov, Vsevolod; Westerman, Eric L.; Hamilton, Frank G.; Walker, David.

In: Journal of Infectious Diseases, Vol. 175, No. 4, 1997, p. 856-863.

Research output: Contribution to journalArticle

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