Genetic and biological characterization of selected Changuinola viruses (Reoviridae, Orbivirus) from Brazil

Sandro P. Silva, Meik Dilcher, Franziska Weber, Frank T. Hufert, Manfred Weidmann, Jedson F. Cardoso, Valéria L. Carvalho, Jannifer O. Chiang, Lívia C. Martins, Clayton P S Lima, Daisy E A Da Silva, João L S G Vianez-Júnior, Vsevolod Popov, Amélia P A Travassos da Rosa, Robert B. Tesh, Pedro F C Vasconcelos, Márcio R T Nunes

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The genus Orbivirus of the family Reoviridae comprises 22 virus species including the Changuinola virus (CGLV) serogroup. The complete genome sequences of 13 CGLV serotypes isolated between 1961 and 1988 from distinct geographical areas of the Brazilian Amazon region were obtained. All viral sequences were obtained from single-passaged CGLV strains grown in Vero cells. CGLVs are the only orbiviruses known to be transmitted by phlebotomine sandflies. Ultrastructure and molecular analysis by electron microscopy and gel electrophoresis, respectively, revealed viral particles with typical orbivirus size and morphology, as well as the presence of a segmented genome with 10 segments. Full-length nucleotide sequencing of each of the ten RNA segments of the 13 CGLV serotypes provided basic information regarding the genome organization, encoded proteins and genetic traits. Segment 2 (encoding VP2) of the CGLV is uncommonly larger in comparison to those found in other orbiviruses and shows varying sizes even among different CGLV serotypes. Phylogenetic analysis support previous serological findings, which indicate that CGLV constitutes a separate serogroup within the genus Orbivirus. In addition, six out of 13 analysed CGLV serotypes showed reassortment of their genome segments.

Original languageEnglish
Pages (from-to)2251-2259
Number of pages9
JournalJournal of General Virology
Volume95
DOIs
StatePublished - Oct 1 2014

Fingerprint

Orbivirus
Reoviridae
Brazil
Genome
Psychodidae
Vero Cells

ASJC Scopus subject areas

  • Virology
  • Medicine(all)

Cite this

Silva, S. P., Dilcher, M., Weber, F., Hufert, F. T., Weidmann, M., Cardoso, J. F., ... Nunes, M. R. T. (2014). Genetic and biological characterization of selected Changuinola viruses (Reoviridae, Orbivirus) from Brazil. Journal of General Virology, 95, 2251-2259. https://doi.org/10.1099/vir.0.064691-0

Genetic and biological characterization of selected Changuinola viruses (Reoviridae, Orbivirus) from Brazil. / Silva, Sandro P.; Dilcher, Meik; Weber, Franziska; Hufert, Frank T.; Weidmann, Manfred; Cardoso, Jedson F.; Carvalho, Valéria L.; Chiang, Jannifer O.; Martins, Lívia C.; Lima, Clayton P S; Da Silva, Daisy E A; Vianez-Júnior, João L S G; Popov, Vsevolod; Travassos da Rosa, Amélia P A; Tesh, Robert B.; Vasconcelos, Pedro F C; Nunes, Márcio R T.

In: Journal of General Virology, Vol. 95, 01.10.2014, p. 2251-2259.

Research output: Contribution to journalArticle

Silva, SP, Dilcher, M, Weber, F, Hufert, FT, Weidmann, M, Cardoso, JF, Carvalho, VL, Chiang, JO, Martins, LC, Lima, CPS, Da Silva, DEA, Vianez-Júnior, JLSG, Popov, V, Travassos da Rosa, APA, Tesh, RB, Vasconcelos, PFC & Nunes, MRT 2014, 'Genetic and biological characterization of selected Changuinola viruses (Reoviridae, Orbivirus) from Brazil', Journal of General Virology, vol. 95, pp. 2251-2259. https://doi.org/10.1099/vir.0.064691-0
Silva, Sandro P. ; Dilcher, Meik ; Weber, Franziska ; Hufert, Frank T. ; Weidmann, Manfred ; Cardoso, Jedson F. ; Carvalho, Valéria L. ; Chiang, Jannifer O. ; Martins, Lívia C. ; Lima, Clayton P S ; Da Silva, Daisy E A ; Vianez-Júnior, João L S G ; Popov, Vsevolod ; Travassos da Rosa, Amélia P A ; Tesh, Robert B. ; Vasconcelos, Pedro F C ; Nunes, Márcio R T. / Genetic and biological characterization of selected Changuinola viruses (Reoviridae, Orbivirus) from Brazil. In: Journal of General Virology. 2014 ; Vol. 95. pp. 2251-2259.
@article{ca1282985b3f46e385e797358149a2ac,
title = "Genetic and biological characterization of selected Changuinola viruses (Reoviridae, Orbivirus) from Brazil",
abstract = "The genus Orbivirus of the family Reoviridae comprises 22 virus species including the Changuinola virus (CGLV) serogroup. The complete genome sequences of 13 CGLV serotypes isolated between 1961 and 1988 from distinct geographical areas of the Brazilian Amazon region were obtained. All viral sequences were obtained from single-passaged CGLV strains grown in Vero cells. CGLVs are the only orbiviruses known to be transmitted by phlebotomine sandflies. Ultrastructure and molecular analysis by electron microscopy and gel electrophoresis, respectively, revealed viral particles with typical orbivirus size and morphology, as well as the presence of a segmented genome with 10 segments. Full-length nucleotide sequencing of each of the ten RNA segments of the 13 CGLV serotypes provided basic information regarding the genome organization, encoded proteins and genetic traits. Segment 2 (encoding VP2) of the CGLV is uncommonly larger in comparison to those found in other orbiviruses and shows varying sizes even among different CGLV serotypes. Phylogenetic analysis support previous serological findings, which indicate that CGLV constitutes a separate serogroup within the genus Orbivirus. In addition, six out of 13 analysed CGLV serotypes showed reassortment of their genome segments.",
author = "Silva, {Sandro P.} and Meik Dilcher and Franziska Weber and Hufert, {Frank T.} and Manfred Weidmann and Cardoso, {Jedson F.} and Carvalho, {Val{\'e}ria L.} and Chiang, {Jannifer O.} and Martins, {L{\'i}via C.} and Lima, {Clayton P S} and {Da Silva}, {Daisy E A} and Vianez-J{\'u}nior, {Jo{\~a}o L S G} and Vsevolod Popov and {Travassos da Rosa}, {Am{\'e}lia P A} and Tesh, {Robert B.} and Vasconcelos, {Pedro F C} and Nunes, {M{\'a}rcio R T}",
year = "2014",
month = "10",
day = "1",
doi = "10.1099/vir.0.064691-0",
language = "English",
volume = "95",
pages = "2251--2259",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",

}

TY - JOUR

T1 - Genetic and biological characterization of selected Changuinola viruses (Reoviridae, Orbivirus) from Brazil

AU - Silva, Sandro P.

AU - Dilcher, Meik

AU - Weber, Franziska

AU - Hufert, Frank T.

AU - Weidmann, Manfred

AU - Cardoso, Jedson F.

AU - Carvalho, Valéria L.

AU - Chiang, Jannifer O.

AU - Martins, Lívia C.

AU - Lima, Clayton P S

AU - Da Silva, Daisy E A

AU - Vianez-Júnior, João L S G

AU - Popov, Vsevolod

AU - Travassos da Rosa, Amélia P A

AU - Tesh, Robert B.

AU - Vasconcelos, Pedro F C

AU - Nunes, Márcio R T

PY - 2014/10/1

Y1 - 2014/10/1

N2 - The genus Orbivirus of the family Reoviridae comprises 22 virus species including the Changuinola virus (CGLV) serogroup. The complete genome sequences of 13 CGLV serotypes isolated between 1961 and 1988 from distinct geographical areas of the Brazilian Amazon region were obtained. All viral sequences were obtained from single-passaged CGLV strains grown in Vero cells. CGLVs are the only orbiviruses known to be transmitted by phlebotomine sandflies. Ultrastructure and molecular analysis by electron microscopy and gel electrophoresis, respectively, revealed viral particles with typical orbivirus size and morphology, as well as the presence of a segmented genome with 10 segments. Full-length nucleotide sequencing of each of the ten RNA segments of the 13 CGLV serotypes provided basic information regarding the genome organization, encoded proteins and genetic traits. Segment 2 (encoding VP2) of the CGLV is uncommonly larger in comparison to those found in other orbiviruses and shows varying sizes even among different CGLV serotypes. Phylogenetic analysis support previous serological findings, which indicate that CGLV constitutes a separate serogroup within the genus Orbivirus. In addition, six out of 13 analysed CGLV serotypes showed reassortment of their genome segments.

AB - The genus Orbivirus of the family Reoviridae comprises 22 virus species including the Changuinola virus (CGLV) serogroup. The complete genome sequences of 13 CGLV serotypes isolated between 1961 and 1988 from distinct geographical areas of the Brazilian Amazon region were obtained. All viral sequences were obtained from single-passaged CGLV strains grown in Vero cells. CGLVs are the only orbiviruses known to be transmitted by phlebotomine sandflies. Ultrastructure and molecular analysis by electron microscopy and gel electrophoresis, respectively, revealed viral particles with typical orbivirus size and morphology, as well as the presence of a segmented genome with 10 segments. Full-length nucleotide sequencing of each of the ten RNA segments of the 13 CGLV serotypes provided basic information regarding the genome organization, encoded proteins and genetic traits. Segment 2 (encoding VP2) of the CGLV is uncommonly larger in comparison to those found in other orbiviruses and shows varying sizes even among different CGLV serotypes. Phylogenetic analysis support previous serological findings, which indicate that CGLV constitutes a separate serogroup within the genus Orbivirus. In addition, six out of 13 analysed CGLV serotypes showed reassortment of their genome segments.

UR - http://www.scopus.com/inward/record.url?scp=84907222900&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907222900&partnerID=8YFLogxK

U2 - 10.1099/vir.0.064691-0

DO - 10.1099/vir.0.064691-0

M3 - Article

VL - 95

SP - 2251

EP - 2259

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

ER -