Genetic and epidemiological studies of dengue type 2 viruses by hybridization using synthetic deoxyoligonucleotides as probes

J. H. Kerschner, A. V. Vorndam, T. P. Monath, D. W. Trent

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A rapid nucleic acid hybridization procedure was developed for examining the genotypic variation of dengue type 2 viruses (DEN 2) having distinct RNase T1 fingerprints and isolated from different geographical areas. Synthetic DNA hybridization probes were constructed complementary in nucleotide sequence to common and unique RNase T1 oligonucleotides of topotype viruses from Puerto Rico/South Pacific, Jamaica, the Seychelles, Thailand/Burma and Africa. Hybridization probes with both type- and topotype-specific reactivities were observed, as were probes specific for two or more of the DEN 2 topotypes. These results confirm geographical movement of topotype virus strains and suggest possible origins. Detection of DEN 2 RNA by hybridization is a rapid and reproducible method that can be modified and applied as a viable alternative to the laborious T1 oligonucleotide fingerprinting.

Original languageEnglish (US)
Pages (from-to)2645-2661
Number of pages17
JournalJournal of General Virology
Volume67
Issue number12
StatePublished - 1986

Fingerprint

Ribonuclease T1
Dengue Virus
Oligonucleotides
Epidemiologic Studies
Seychelles
Myanmar
Viruses
Nucleic Acid Hybridization
Jamaica
Puerto Rico
DNA Probes
Dermatoglyphics
Thailand
RNA

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Genetic and epidemiological studies of dengue type 2 viruses by hybridization using synthetic deoxyoligonucleotides as probes. / Kerschner, J. H.; Vorndam, A. V.; Monath, T. P.; Trent, D. W.

In: Journal of General Virology, Vol. 67, No. 12, 1986, p. 2645-2661.

Research output: Contribution to journalArticle

Kerschner, J. H. ; Vorndam, A. V. ; Monath, T. P. ; Trent, D. W. / Genetic and epidemiological studies of dengue type 2 viruses by hybridization using synthetic deoxyoligonucleotides as probes. In: Journal of General Virology. 1986 ; Vol. 67, No. 12. pp. 2645-2661.
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