Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains

Shannan Rossi, F. Nasar, J. Cardosa, S. V. Mayer, R. B. Tesh, K. A. Hanley, Scott Weaver, Nikos Vasilakis

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Four serotypes of dengue virus (DENV 1-4) currently circulate between humans and domestic/peridomestic Aedes mosquitoes, resulting in 100. million infections per year. All four serotypes emerged, independently, from sylvatic progenitors transmitted among non-human primates by arboreal Aedes mosquitoes. This study investigated the genetic and phenotypic changes associated with emergence of human DENV-4 from its sylvatic ancestors. Analysis of complete genomes of 3 sylvatic and 4 human strains revealed high conservation of both the 5'- and 3'-untranslated regions but considerable divergence within the open reading frame. Additionally, the two ecotypes did not differ significantly in replication dynamics in cultured human liver (Huh-7), monkey kidney (Vero) or mosquito (C6/36) cells, although significant inter-strain variation within ecotypes was detected. These findings are in partial agreement with previous studies of DENV-2, where human strains produced a larger number of progeny than sylvatic strains in human liver cells but not in monkey or mosquito cells.

Original languageEnglish (US)
Pages (from-to)58-67
Number of pages10
JournalVirology
Volume423
Issue number1
DOIs
StatePublished - Feb 5 2012

Fingerprint

Dengue Virus
Culicidae
Ecotype
Aedes
Haplorhini
5' Untranslated Regions
Liver
3' Untranslated Regions
Primates
Open Reading Frames
Genome
Kidney
Infection

Keywords

  • Dengue virus (DENV)
  • Human DENV
  • Phylogenetic and phenotypic analyses
  • Sylvatic DENV

ASJC Scopus subject areas

  • Virology

Cite this

Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains. / Rossi, Shannan; Nasar, F.; Cardosa, J.; Mayer, S. V.; Tesh, R. B.; Hanley, K. A.; Weaver, Scott; Vasilakis, Nikos.

In: Virology, Vol. 423, No. 1, 05.02.2012, p. 58-67.

Research output: Contribution to journalArticle

Rossi, Shannan ; Nasar, F. ; Cardosa, J. ; Mayer, S. V. ; Tesh, R. B. ; Hanley, K. A. ; Weaver, Scott ; Vasilakis, Nikos. / Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains. In: Virology. 2012 ; Vol. 423, No. 1. pp. 58-67.
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