Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains

S. L. Rossi, F. Nasar, J. Cardosa, S. V. Mayer, R. B. Tesh, K. A. Hanley, S. C. Weaver, Nikos Vasilakis

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Four serotypes of dengue virus (DENV 1-4) currently circulate between humans and domestic/peridomestic Aedes mosquitoes, resulting in 100. million infections per year. All four serotypes emerged, independently, from sylvatic progenitors transmitted among non-human primates by arboreal Aedes mosquitoes. This study investigated the genetic and phenotypic changes associated with emergence of human DENV-4 from its sylvatic ancestors. Analysis of complete genomes of 3 sylvatic and 4 human strains revealed high conservation of both the 5'- and 3'-untranslated regions but considerable divergence within the open reading frame. Additionally, the two ecotypes did not differ significantly in replication dynamics in cultured human liver (Huh-7), monkey kidney (Vero) or mosquito (C6/36) cells, although significant inter-strain variation within ecotypes was detected. These findings are in partial agreement with previous studies of DENV-2, where human strains produced a larger number of progeny than sylvatic strains in human liver cells but not in monkey or mosquito cells.

Original languageEnglish (US)
Pages (from-to)58-67
Number of pages10
JournalVirology
Volume423
Issue number1
DOIs
StatePublished - Feb 5 2012

Keywords

  • Dengue virus (DENV)
  • Human DENV
  • Phylogenetic and phenotypic analyses
  • Sylvatic DENV

ASJC Scopus subject areas

  • Virology

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