Genetic and phenotypic characterization of sylvatic dengue virus type 2 strains

Nikos Vasilakis, Eric B. Fokam, Christopher T. Hanson, Ethan Weinberg, Amadou A. Sall, Stephen S. Whitehead, Kathryn A. Hanley, Scott Weaver

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The four serotypes of endemic dengue viruses (DENV) circulate between humans and peridomestic Aedes mosquitoes. At present endemic DENV infect 100 million people per year, and a third of the global population is at risk. In contrast, sylvatic DENV strains are maintained in a transmission cycle between nonhuman primates and sylvatic Aedes species, and are evolutionarily and ecologically distinct from endemic DENV strains. Phylogenetic analyses place sylvatic strains basal to each of the endemic serotypes, supporting the hypothesis that each of the endemic DENV serotypes emerged independently from sylvatic ancestors. We utilized complete genome analyses of both sylvatic and endemic DENV serotype 2 (DENV-2) to expand our understanding of their genetic relationships. A high degree of conservation was observed in both the 5′- and 3′-untranslated genome regions, whereas considerable differences at the nucleotide and amino acid levels were observed within the open reading frame. Additionally, replication of the two genotypes was compared in cultured cells, where endemic DENV strains produced a significantly higher output of progeny in human liver cells, but not in monkey kidney or mosquito cells. Understanding the genetic relationships and phenotypic differences between endemic and sylvatic DENV genotypes may provide valuable insight into DENV emergence and guide monitoring of future outbreaks.

Original languageEnglish (US)
Pages (from-to)296-307
Number of pages12
JournalVirology
Volume377
Issue number2
DOIs
StatePublished - Aug 1 2008

Fingerprint

Dengue Virus
Aedes
Culicidae
Genotype
Genome
3' Untranslated Regions
Primates
Open Reading Frames
Haplorhini
Disease Outbreaks
Cultured Cells
Nucleotides
Kidney
Amino Acids
Serogroup
Liver

Keywords

  • Dengue virus (DENV)
  • Endemic DENV
  • Phylogenetic and phenotypic analysis
  • Sylvatic DENV

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Genetic and phenotypic characterization of sylvatic dengue virus type 2 strains. / Vasilakis, Nikos; Fokam, Eric B.; Hanson, Christopher T.; Weinberg, Ethan; Sall, Amadou A.; Whitehead, Stephen S.; Hanley, Kathryn A.; Weaver, Scott.

In: Virology, Vol. 377, No. 2, 01.08.2008, p. 296-307.

Research output: Contribution to journalArticle

Vasilakis, N, Fokam, EB, Hanson, CT, Weinberg, E, Sall, AA, Whitehead, SS, Hanley, KA & Weaver, S 2008, 'Genetic and phenotypic characterization of sylvatic dengue virus type 2 strains', Virology, vol. 377, no. 2, pp. 296-307. https://doi.org/10.1016/j.virol.2008.04.044
Vasilakis N, Fokam EB, Hanson CT, Weinberg E, Sall AA, Whitehead SS et al. Genetic and phenotypic characterization of sylvatic dengue virus type 2 strains. Virology. 2008 Aug 1;377(2):296-307. https://doi.org/10.1016/j.virol.2008.04.044
Vasilakis, Nikos ; Fokam, Eric B. ; Hanson, Christopher T. ; Weinberg, Ethan ; Sall, Amadou A. ; Whitehead, Stephen S. ; Hanley, Kathryn A. ; Weaver, Scott. / Genetic and phenotypic characterization of sylvatic dengue virus type 2 strains. In: Virology. 2008 ; Vol. 377, No. 2. pp. 296-307.
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