TY - JOUR
T1 - Genetic characterization, molecular epidemiology, and phylogenetic relationships of insect-specific viruses in the taxon Negevirus
AU - Nunes, Marcio R.T.
AU - da Silva, Sandro Patroca
AU - Cardoso, Jedson F.
AU - de Oliveira, Rodrigo Santo
AU - da Silva Lemos, Poliana
AU - Vasconcelos, Pedro F.C.
AU - Vasilakis, Nikos
AU - Tesh, Robert B.
N1 - Funding Information:
Work at UTMB was supported by NIH grant U54 AI057156, R24 AI120942 (HG, RBT, NV) and by NIH contract HHSN272202000040I/HHSN200004/D04 (HG, RBT, NV). MAC was supported by Programa de Doctorados Nacionales – Colciencias” (Convocatoria 567) (MAC) PhD fellowship. The field collection in Colombia was supported in part by Colciencias C.T-695-2014 and by Colciencias grant 111549326198. This work was supported in part by CNPq (Brazilian National Council for Research and Development) grant no. 302032/2011-8 (MRTN). We also acknowledge support from the Yale Institute for Biospheric Studies Center for EcoEpidemiology and from the U.S. National Park Service (Collecting Permit #EVER-2013-SCI-0032). We thank to Dr. Juan Carlos Gallego-Gómez and Richard Hoyos from Universidad de Antioquia for assistance in field collections and entomological processing of the samples in Colombia. Joao L. Vianez for contributing the box plot analyses.
Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2017/4/1
Y1 - 2017/4/1
N2 - The recently described taxon Negevirus is comprised of a diverse group of insect-specific viruses isolated from mosquitoes and phlebotomine sandflies. In this study, a comprehensive genetic characterization, molecular, epidemiological and evolutionary analyses were conducted on nearly full-length sequences of 91 new negevirus isolates obtained in Brazil, Colombia, Peru, Panama, USA and Nepal. We demonstrated that these arthropod restricted viruses are clustered in two major phylogenetic groups with origins related to three plant virus genera (Cilevirus, Higrevirus and Blunevirus). Molecular analyses demonstrated that specific host correlations are not present with most negeviruses; instead, high genetic variability, wide host-range, and cross-species transmission were noted. The data presented here also revealed the existence of five novel insect-specific viruses falling into two arthropod-restrictive virus taxa, previously proposed as distinct genera, designated Nelorpivirus and Sandewavirus. Our results provide a better understanding of the molecular epidemiology, evolution, taxonomy and stability of this group of insect-restricted viruses.
AB - The recently described taxon Negevirus is comprised of a diverse group of insect-specific viruses isolated from mosquitoes and phlebotomine sandflies. In this study, a comprehensive genetic characterization, molecular, epidemiological and evolutionary analyses were conducted on nearly full-length sequences of 91 new negevirus isolates obtained in Brazil, Colombia, Peru, Panama, USA and Nepal. We demonstrated that these arthropod restricted viruses are clustered in two major phylogenetic groups with origins related to three plant virus genera (Cilevirus, Higrevirus and Blunevirus). Molecular analyses demonstrated that specific host correlations are not present with most negeviruses; instead, high genetic variability, wide host-range, and cross-species transmission were noted. The data presented here also revealed the existence of five novel insect-specific viruses falling into two arthropod-restrictive virus taxa, previously proposed as distinct genera, designated Nelorpivirus and Sandewavirus. Our results provide a better understanding of the molecular epidemiology, evolution, taxonomy and stability of this group of insect-restricted viruses.
KW - Insect-specific viruses
KW - Negevirus
KW - Nelorpivirus genus
KW - Sandewavirus genus
KW - Virus diversity
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U2 - 10.1016/j.virol.2017.01.022
DO - 10.1016/j.virol.2017.01.022
M3 - Article
C2 - 28193550
AN - SCOPUS:85012154572
VL - 504
SP - 152
EP - 167
JO - Virology
JF - Virology
SN - 0042-6822
ER -