Genetic characterization, molecular epidemiology, and phylogenetic relationships of insect-specific viruses in the taxon Negevirus

Marcio R T Nunes, Sandro Patroca da Silva, Jedson F. Cardoso, Rodrigo Santo de Oliveira, Poliana da Silva Lemos, Pedro F C Vasconcelos, Nikos Vasilakis, Robert B. Tesh

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The recently described taxon Negevirus is comprised of a diverse group of insect-specific viruses isolated from mosquitoes and phlebotomine sandflies. In this study, a comprehensive genetic characterization, molecular, epidemiological and evolutionary analyses were conducted on nearly full-length sequences of 91 new negevirus isolates obtained in Brazil, Colombia, Peru, Panama, USA and Nepal. We demonstrated that these arthropod restricted viruses are clustered in two major phylogenetic groups with origins related to three plant virus genera (Cilevirus, Higrevirus and Blunevirus). Molecular analyses demonstrated that specific host correlations are not present with most negeviruses; instead, high genetic variability, wide host-range, and cross-species transmission were noted. The data presented here also revealed the existence of five novel insect-specific viruses falling into two arthropod-restrictive virus taxa, previously proposed as distinct genera, designated Nelorpivirus and Sandewavirus. Our results provide a better understanding of the molecular epidemiology, evolution, taxonomy and stability of this group of insect-restricted viruses.

Original languageEnglish (US)
Pages (from-to)152-167
Number of pages16
JournalVirology
Volume504
DOIs
StatePublished - Apr 1 2017
Externally publishedYes

Fingerprint

Insect Viruses
Molecular Epidemiology
Arthropods
Viruses
Panama
Plant Viruses
Psychodidae
Nepal
Peru
Colombia
Molecular Evolution
Host Specificity
Culicidae
Brazil
Molecular Biology

Keywords

  • Insect-specific viruses
  • Negevirus
  • Nelorpivirus genus
  • Sandewavirus genus
  • Virus diversity

ASJC Scopus subject areas

  • Virology

Cite this

Nunes, M. R. T., da Silva, S. P., Cardoso, J. F., de Oliveira, R. S., da Silva Lemos, P., Vasconcelos, P. F. C., ... Tesh, R. B. (2017). Genetic characterization, molecular epidemiology, and phylogenetic relationships of insect-specific viruses in the taxon Negevirus. Virology, 504, 152-167. https://doi.org/10.1016/j.virol.2017.01.022

Genetic characterization, molecular epidemiology, and phylogenetic relationships of insect-specific viruses in the taxon Negevirus. / Nunes, Marcio R T; da Silva, Sandro Patroca; Cardoso, Jedson F.; de Oliveira, Rodrigo Santo; da Silva Lemos, Poliana; Vasconcelos, Pedro F C; Vasilakis, Nikos; Tesh, Robert B.

In: Virology, Vol. 504, 01.04.2017, p. 152-167.

Research output: Contribution to journalArticle

Nunes, MRT, da Silva, SP, Cardoso, JF, de Oliveira, RS, da Silva Lemos, P, Vasconcelos, PFC, Vasilakis, N & Tesh, RB 2017, 'Genetic characterization, molecular epidemiology, and phylogenetic relationships of insect-specific viruses in the taxon Negevirus', Virology, vol. 504, pp. 152-167. https://doi.org/10.1016/j.virol.2017.01.022
Nunes MRT, da Silva SP, Cardoso JF, de Oliveira RS, da Silva Lemos P, Vasconcelos PFC et al. Genetic characterization, molecular epidemiology, and phylogenetic relationships of insect-specific viruses in the taxon Negevirus. Virology. 2017 Apr 1;504:152-167. https://doi.org/10.1016/j.virol.2017.01.022
Nunes, Marcio R T ; da Silva, Sandro Patroca ; Cardoso, Jedson F. ; de Oliveira, Rodrigo Santo ; da Silva Lemos, Poliana ; Vasconcelos, Pedro F C ; Vasilakis, Nikos ; Tesh, Robert B. / Genetic characterization, molecular epidemiology, and phylogenetic relationships of insect-specific viruses in the taxon Negevirus. In: Virology. 2017 ; Vol. 504. pp. 152-167.
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