Abstract
The recently described taxon Negevirus is comprised of a diverse group of insect-specific viruses isolated from mosquitoes and phlebotomine sandflies. In this study, a comprehensive genetic characterization, molecular, epidemiological and evolutionary analyses were conducted on nearly full-length sequences of 91 new negevirus isolates obtained in Brazil, Colombia, Peru, Panama, USA and Nepal. We demonstrated that these arthropod restricted viruses are clustered in two major phylogenetic groups with origins related to three plant virus genera (Cilevirus, Higrevirus and Blunevirus). Molecular analyses demonstrated that specific host correlations are not present with most negeviruses; instead, high genetic variability, wide host-range, and cross-species transmission were noted. The data presented here also revealed the existence of five novel insect-specific viruses falling into two arthropod-restrictive virus taxa, previously proposed as distinct genera, designated Nelorpivirus and Sandewavirus. Our results provide a better understanding of the molecular epidemiology, evolution, taxonomy and stability of this group of insect-restricted viruses.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 152-167 |
| Number of pages | 16 |
| Journal | Virology |
| Volume | 504 |
| DOIs | |
| State | Published - Apr 1 2017 |
| Externally published | Yes |
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Keywords
- Insect-specific viruses
- Negevirus
- Nelorpivirus genus
- Sandewavirus genus
- Virus diversity
ASJC Scopus subject areas
- Virology
Cite this
Genetic characterization, molecular epidemiology, and phylogenetic relationships of insect-specific viruses in the taxon Negevirus. / Nunes, Marcio R T; da Silva, Sandro Patroca; Cardoso, Jedson F.; de Oliveira, Rodrigo Santo; da Silva Lemos, Poliana; Vasconcelos, Pedro F C; Vasilakis, Nikos; Tesh, Robert B.
In: Virology, Vol. 504, 01.04.2017, p. 152-167.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genetic characterization, molecular epidemiology, and phylogenetic relationships of insect-specific viruses in the taxon Negevirus
AU - Nunes, Marcio R T
AU - da Silva, Sandro Patroca
AU - Cardoso, Jedson F.
AU - de Oliveira, Rodrigo Santo
AU - da Silva Lemos, Poliana
AU - Vasconcelos, Pedro F C
AU - Vasilakis, Nikos
AU - Tesh, Robert B.
PY - 2017/4/1
Y1 - 2017/4/1
N2 - The recently described taxon Negevirus is comprised of a diverse group of insect-specific viruses isolated from mosquitoes and phlebotomine sandflies. In this study, a comprehensive genetic characterization, molecular, epidemiological and evolutionary analyses were conducted on nearly full-length sequences of 91 new negevirus isolates obtained in Brazil, Colombia, Peru, Panama, USA and Nepal. We demonstrated that these arthropod restricted viruses are clustered in two major phylogenetic groups with origins related to three plant virus genera (Cilevirus, Higrevirus and Blunevirus). Molecular analyses demonstrated that specific host correlations are not present with most negeviruses; instead, high genetic variability, wide host-range, and cross-species transmission were noted. The data presented here also revealed the existence of five novel insect-specific viruses falling into two arthropod-restrictive virus taxa, previously proposed as distinct genera, designated Nelorpivirus and Sandewavirus. Our results provide a better understanding of the molecular epidemiology, evolution, taxonomy and stability of this group of insect-restricted viruses.
AB - The recently described taxon Negevirus is comprised of a diverse group of insect-specific viruses isolated from mosquitoes and phlebotomine sandflies. In this study, a comprehensive genetic characterization, molecular, epidemiological and evolutionary analyses were conducted on nearly full-length sequences of 91 new negevirus isolates obtained in Brazil, Colombia, Peru, Panama, USA and Nepal. We demonstrated that these arthropod restricted viruses are clustered in two major phylogenetic groups with origins related to three plant virus genera (Cilevirus, Higrevirus and Blunevirus). Molecular analyses demonstrated that specific host correlations are not present with most negeviruses; instead, high genetic variability, wide host-range, and cross-species transmission were noted. The data presented here also revealed the existence of five novel insect-specific viruses falling into two arthropod-restrictive virus taxa, previously proposed as distinct genera, designated Nelorpivirus and Sandewavirus. Our results provide a better understanding of the molecular epidemiology, evolution, taxonomy and stability of this group of insect-restricted viruses.
KW - Insect-specific viruses
KW - Negevirus
KW - Nelorpivirus genus
KW - Sandewavirus genus
KW - Virus diversity
UR - http://www.scopus.com/inward/record.url?scp=85012154572&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85012154572&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2017.01.022
DO - 10.1016/j.virol.2017.01.022
M3 - Article
C2 - 28193550
AN - SCOPUS:85012154572
VL - 504
SP - 152
EP - 167
JO - Virology
JF - Virology
SN - 0042-6822
ER -