Genetic characterization of northwestern Colombian chikungunya virus strains from the 2014-2015 epidemic

Juan D. Rodas, Tiffany Kautz, Erwin Camacho, Luis Paternina, Hilda Guzmán, Francisco J. Díaz, Pedro Blanco, Robert Tesh, Scott Weaver

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Chikungunya fever, an acute and often chronic arthralgic disease caused by the mosquito-borne alphavirus, chikungunya virus (CHIKV), spread into the Americas in late 2013. Since then it has caused epidemics in nearly all New World countries, the second largest being Colombia with over 450,000 suspected cases beginning in September, 2014, and focused in Bolivar Department in the north. We examined 32 human sera from suspected cases, including diverse age groups and both genders, and sequenced the CHIKV envelope glycoprotein genes, known determinants of vector host range. As expected for Asian lineage CHIKV strains, these isolates lacked known Aedes albopictus-adaptive mutations. All the Colombian strains were closely related to those from the Virgin Islands, Saint Lucia, Mexico, Puerto Rico, and Brazil, consistent with a single, point-source introduction from the southeast Asia/Pacific region. Two substitutions in the E2 and E1 envelope glycoprotein genes were found in the Colombian strains, especially E1-K211E involving a residue shown previously to affect epistatically the penetrance of the E1-A226V A. albopictus-adaptive substitution. We also identified two amino acid substitutions unique to all American CHIKV sequences: E2-V368A and 6K-L20M. Only one codon, 6K-47, had a high nonsynonymous substitution rate suggesting positive selection.

Original languageEnglish (US)
Pages (from-to)639-646
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume95
Issue number3
DOIs
StatePublished - Sep 1 2016

Fingerprint

Chikungunya virus
Saint Lucia
Glycoproteins
Alphavirus
Puerto Rico
Southeastern Asia
Colombia
Penetrance
Aedes
Host Specificity
Amino Acid Substitution
Mexico
Culicidae
Islands
Codon
Genes
Brazil
Chronic Disease
Age Groups
Mutation

ASJC Scopus subject areas

  • Parasitology
  • Medicine(all)
  • Virology
  • Infectious Diseases

Cite this

Genetic characterization of northwestern Colombian chikungunya virus strains from the 2014-2015 epidemic. / Rodas, Juan D.; Kautz, Tiffany; Camacho, Erwin; Paternina, Luis; Guzmán, Hilda; Díaz, Francisco J.; Blanco, Pedro; Tesh, Robert; Weaver, Scott.

In: American Journal of Tropical Medicine and Hygiene, Vol. 95, No. 3, 01.09.2016, p. 639-646.

Research output: Contribution to journalArticle

Rodas, Juan D. ; Kautz, Tiffany ; Camacho, Erwin ; Paternina, Luis ; Guzmán, Hilda ; Díaz, Francisco J. ; Blanco, Pedro ; Tesh, Robert ; Weaver, Scott. / Genetic characterization of northwestern Colombian chikungunya virus strains from the 2014-2015 epidemic. In: American Journal of Tropical Medicine and Hygiene. 2016 ; Vol. 95, No. 3. pp. 639-646.
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