Abstract
The International Committee for Taxonomy of Viruses (ICTV) recognizes four species of tick-borne orbiviruses (TBOs): Chenuda virus, Chobar Gorge virus, Wad Medani virus and Great Island virus (genus Orbivirus, family Reoviridae). Nucleotide (nt) and amino acid (aa) sequence comparisons provide a basis for orbivirus detection and classification, however full genome sequence data were only available for the Great Island virus species. We report representative genome-sequences for the three other TBO species (virus isolates: Chenuda virus (CNUV); Chobar Gorge virus (CGV) and Wad Medani virus (WMV)). Phylogenetic comparisons show that TBOs cluster separately from insect-borne orbiviruses (IBOs). CNUV, CGV, WMV and GIV share low level aa/nt identities with other orbiviruses, in ‘conserved’ Pol, T2 and T13 proteins/genes, identifying them as four distinct virus-species. The TBO genome segment encoding cell attachment, outer capsid protein 1 (OC1), is approximately half the size of the equivalent segment from insect-borne orbiviruses, helping to explain why tick-borne orbiviruses have a ~1 kb smaller genome.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2185-2209 |
| Number of pages | 25 |
| Journal | Viruses |
| Volume | 7 |
| Issue number | 5 |
| DOIs | |
| State | Published - Apr 28 2015 |
| Externally published | Yes |
Keywords
- Chenuda virus
- Chobar Gorge virus
- DsRNA virus
- Full genome
- Great Island virus
- Kemorovo virus
- Orbivirus
- Reoviridae
- Sequencing
- Wad medani virus
ASJC Scopus subject areas
- Infectious Diseases
- Virology