Genetic characterization of the tick-borne orbiviruses

Manjunatha N. Belaganahalli, Sushila Maan, Narender S. Maan, Joe Brownlie, Robert Tesh, Houssam Attoui, Peter P C Mertens

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The International Committee for Taxonomy of Viruses (ICTV) recognizes four species of tick-borne orbiviruses (TBOs): Chenuda virus, Chobar Gorge virus, Wad Medani virus and Great Island virus (genus Orbivirus, family Reoviridae). Nucleotide (nt) and amino acid (aa) sequence comparisons provide a basis for orbivirus detection and classification, however full genome sequence data were only available for the Great Island virus species. We report representative genome-sequences for the three other TBO species (virus isolates: Chenuda virus (CNUV); Chobar Gorge virus (CGV) and Wad Medani virus (WMV)). Phylogenetic comparisons show that TBOs cluster separately from insect-borne orbiviruses (IBOs). CNUV, CGV, WMV and GIV share low level aa/nt identities with other orbiviruses, in ‘conserved’ Pol, T2 and T13 proteins/genes, identifying them as four distinct virus-species. The TBO genome segment encoding cell attachment, outer capsid protein 1 (OC1), is approximately half the size of the equivalent segment from insect-borne orbiviruses, helping to explain why tick-borne orbiviruses have a ~1 kb smaller genome.

Original languageEnglish (US)
Pages (from-to)2185-2209
Number of pages25
JournalViruses
Volume7
Issue number5
DOIs
StatePublished - Apr 28 2015

Fingerprint

Orbivirus
Ticks
Viruses
Genome
Islands
Insects
Nucleotides
Reoviridae
Capsid Proteins

Keywords

  • Chenuda virus
  • Chobar Gorge virus
  • DsRNA virus
  • Full genome
  • Great Island virus
  • Kemorovo virus
  • Orbivirus
  • Reoviridae
  • Sequencing
  • Wad medani virus

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Belaganahalli, M. N., Maan, S., Maan, N. S., Brownlie, J., Tesh, R., Attoui, H., & Mertens, P. P. C. (2015). Genetic characterization of the tick-borne orbiviruses. Viruses, 7(5), 2185-2209. https://doi.org/10.3390/v7052185

Genetic characterization of the tick-borne orbiviruses. / Belaganahalli, Manjunatha N.; Maan, Sushila; Maan, Narender S.; Brownlie, Joe; Tesh, Robert; Attoui, Houssam; Mertens, Peter P C.

In: Viruses, Vol. 7, No. 5, 28.04.2015, p. 2185-2209.

Research output: Contribution to journalArticle

Belaganahalli, MN, Maan, S, Maan, NS, Brownlie, J, Tesh, R, Attoui, H & Mertens, PPC 2015, 'Genetic characterization of the tick-borne orbiviruses', Viruses, vol. 7, no. 5, pp. 2185-2209. https://doi.org/10.3390/v7052185
Belaganahalli MN, Maan S, Maan NS, Brownlie J, Tesh R, Attoui H et al. Genetic characterization of the tick-borne orbiviruses. Viruses. 2015 Apr 28;7(5):2185-2209. https://doi.org/10.3390/v7052185
Belaganahalli, Manjunatha N. ; Maan, Sushila ; Maan, Narender S. ; Brownlie, Joe ; Tesh, Robert ; Attoui, Houssam ; Mertens, Peter P C. / Genetic characterization of the tick-borne orbiviruses. In: Viruses. 2015 ; Vol. 7, No. 5. pp. 2185-2209.
@article{733630c209e04c78ad18fe5eef7facd8,
title = "Genetic characterization of the tick-borne orbiviruses",
abstract = "The International Committee for Taxonomy of Viruses (ICTV) recognizes four species of tick-borne orbiviruses (TBOs): Chenuda virus, Chobar Gorge virus, Wad Medani virus and Great Island virus (genus Orbivirus, family Reoviridae). Nucleotide (nt) and amino acid (aa) sequence comparisons provide a basis for orbivirus detection and classification, however full genome sequence data were only available for the Great Island virus species. We report representative genome-sequences for the three other TBO species (virus isolates: Chenuda virus (CNUV); Chobar Gorge virus (CGV) and Wad Medani virus (WMV)). Phylogenetic comparisons show that TBOs cluster separately from insect-borne orbiviruses (IBOs). CNUV, CGV, WMV and GIV share low level aa/nt identities with other orbiviruses, in ‘conserved’ Pol, T2 and T13 proteins/genes, identifying them as four distinct virus-species. The TBO genome segment encoding cell attachment, outer capsid protein 1 (OC1), is approximately half the size of the equivalent segment from insect-borne orbiviruses, helping to explain why tick-borne orbiviruses have a ~1 kb smaller genome.",
keywords = "Chenuda virus, Chobar Gorge virus, DsRNA virus, Full genome, Great Island virus, Kemorovo virus, Orbivirus, Reoviridae, Sequencing, Wad medani virus",
author = "Belaganahalli, {Manjunatha N.} and Sushila Maan and Maan, {Narender S.} and Joe Brownlie and Robert Tesh and Houssam Attoui and Mertens, {Peter P C}",
year = "2015",
month = "4",
day = "28",
doi = "10.3390/v7052185",
language = "English (US)",
volume = "7",
pages = "2185--2209",
journal = "Viruses",
issn = "1999-4915",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "5",

}

TY - JOUR

T1 - Genetic characterization of the tick-borne orbiviruses

AU - Belaganahalli, Manjunatha N.

AU - Maan, Sushila

AU - Maan, Narender S.

AU - Brownlie, Joe

AU - Tesh, Robert

AU - Attoui, Houssam

AU - Mertens, Peter P C

PY - 2015/4/28

Y1 - 2015/4/28

N2 - The International Committee for Taxonomy of Viruses (ICTV) recognizes four species of tick-borne orbiviruses (TBOs): Chenuda virus, Chobar Gorge virus, Wad Medani virus and Great Island virus (genus Orbivirus, family Reoviridae). Nucleotide (nt) and amino acid (aa) sequence comparisons provide a basis for orbivirus detection and classification, however full genome sequence data were only available for the Great Island virus species. We report representative genome-sequences for the three other TBO species (virus isolates: Chenuda virus (CNUV); Chobar Gorge virus (CGV) and Wad Medani virus (WMV)). Phylogenetic comparisons show that TBOs cluster separately from insect-borne orbiviruses (IBOs). CNUV, CGV, WMV and GIV share low level aa/nt identities with other orbiviruses, in ‘conserved’ Pol, T2 and T13 proteins/genes, identifying them as four distinct virus-species. The TBO genome segment encoding cell attachment, outer capsid protein 1 (OC1), is approximately half the size of the equivalent segment from insect-borne orbiviruses, helping to explain why tick-borne orbiviruses have a ~1 kb smaller genome.

AB - The International Committee for Taxonomy of Viruses (ICTV) recognizes four species of tick-borne orbiviruses (TBOs): Chenuda virus, Chobar Gorge virus, Wad Medani virus and Great Island virus (genus Orbivirus, family Reoviridae). Nucleotide (nt) and amino acid (aa) sequence comparisons provide a basis for orbivirus detection and classification, however full genome sequence data were only available for the Great Island virus species. We report representative genome-sequences for the three other TBO species (virus isolates: Chenuda virus (CNUV); Chobar Gorge virus (CGV) and Wad Medani virus (WMV)). Phylogenetic comparisons show that TBOs cluster separately from insect-borne orbiviruses (IBOs). CNUV, CGV, WMV and GIV share low level aa/nt identities with other orbiviruses, in ‘conserved’ Pol, T2 and T13 proteins/genes, identifying them as four distinct virus-species. The TBO genome segment encoding cell attachment, outer capsid protein 1 (OC1), is approximately half the size of the equivalent segment from insect-borne orbiviruses, helping to explain why tick-borne orbiviruses have a ~1 kb smaller genome.

KW - Chenuda virus

KW - Chobar Gorge virus

KW - DsRNA virus

KW - Full genome

KW - Great Island virus

KW - Kemorovo virus

KW - Orbivirus

KW - Reoviridae

KW - Sequencing

KW - Wad medani virus

UR - http://www.scopus.com/inward/record.url?scp=84929359530&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929359530&partnerID=8YFLogxK

U2 - 10.3390/v7052185

DO - 10.3390/v7052185

M3 - Article

C2 - 25928203

AN - SCOPUS:84929359530

VL - 7

SP - 2185

EP - 2209

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 5

ER -