Genetic characterization of the tick-borne orbiviruses

Manjunatha N. Belaganahalli, Sushila Maan, Narender S. Maan, Joe Brownlie, Robert Tesh, Houssam Attoui, Peter P.C. Mertens

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The International Committee for Taxonomy of Viruses (ICTV) recognizes four species of tick-borne orbiviruses (TBOs): Chenuda virus, Chobar Gorge virus, Wad Medani virus and Great Island virus (genus Orbivirus, family Reoviridae). Nucleotide (nt) and amino acid (aa) sequence comparisons provide a basis for orbivirus detection and classification, however full genome sequence data were only available for the Great Island virus species. We report representative genome-sequences for the three other TBO species (virus isolates: Chenuda virus (CNUV); Chobar Gorge virus (CGV) and Wad Medani virus (WMV)). Phylogenetic comparisons show that TBOs cluster separately from insect-borne orbiviruses (IBOs). CNUV, CGV, WMV and GIV share low level aa/nt identities with other orbiviruses, in ‘conserved’ Pol, T2 and T13 proteins/genes, identifying them as four distinct virus-species. The TBO genome segment encoding cell attachment, outer capsid protein 1 (OC1), is approximately half the size of the equivalent segment from insect-borne orbiviruses, helping to explain why tick-borne orbiviruses have a ~1 kb smaller genome.

Original languageEnglish (US)
Pages (from-to)2185-2209
Number of pages25
JournalViruses
Volume7
Issue number5
DOIs
StatePublished - Apr 28 2015

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Keywords

  • Chenuda virus
  • Chobar Gorge virus
  • DsRNA virus
  • Full genome
  • Great Island virus
  • Kemorovo virus
  • Orbivirus
  • Reoviridae
  • Sequencing
  • Wad medani virus

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Belaganahalli, M. N., Maan, S., Maan, N. S., Brownlie, J., Tesh, R., Attoui, H., & Mertens, P. P. C. (2015). Genetic characterization of the tick-borne orbiviruses. Viruses, 7(5), 2185-2209. https://doi.org/10.3390/v7052185