Genetic classification of 'Rickettsia heilongjiangii' and 'Rickettsia hulinii,' two chinese spotted fever group rickettsiae

Jianzhi Zhang, M. Y. Fan, Y. M. Wu, P. Fournier, V. Roux, D. Raoult

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

To determine the phylogenetic position of two new rickettsial strains isolated from ticks in China, 16S ribosomal DNA, gltA, and ompA (apart from the tandem repeat units) genes were amplified by PCR and sequenced. The phylogenetic relationships between these strains and other rickettsiae were inferred from the comparison of sequences of the three genes by the parsimony, neighbor-joining, and maximum-likelihood methods. The results demonstrated that the 054 strain, a rickettsia pathogenic in humans, and the HL-93 strain were related and clustered together with Rickettsia japonica. Significant statistical bootstrap values (100 and 92%) supported the nodes in this cluster. Based on previous genotypic and antigenic data and the phylogenetic analysis presented here, the 054 and HL-93 strains should be considered as new species, and we formally propose that they be named 'Rickettsia heilongjiangii' and 'Rickettsia hulinii,' respectively.

Original languageEnglish (US)
Pages (from-to)3498-3501
Number of pages4
JournalJournal of Clinical Microbiology
Volume38
Issue number9
StatePublished - 2000
Externally publishedYes

Fingerprint

Rickettsia
Fever
Tandem Repeat Sequences
Ticks
Ribosomal DNA
Genes
China
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Genetic classification of 'Rickettsia heilongjiangii' and 'Rickettsia hulinii,' two chinese spotted fever group rickettsiae. / Zhang, Jianzhi; Fan, M. Y.; Wu, Y. M.; Fournier, P.; Roux, V.; Raoult, D.

In: Journal of Clinical Microbiology, Vol. 38, No. 9, 2000, p. 3498-3501.

Research output: Contribution to journalArticle

Zhang, Jianzhi ; Fan, M. Y. ; Wu, Y. M. ; Fournier, P. ; Roux, V. ; Raoult, D. / Genetic classification of 'Rickettsia heilongjiangii' and 'Rickettsia hulinii,' two chinese spotted fever group rickettsiae. In: Journal of Clinical Microbiology. 2000 ; Vol. 38, No. 9. pp. 3498-3501.
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AU - Raoult, D.

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