Genetic diversification and recombination of HIV type 1 group M in Kinshasa, Democratic Republic of Congo

Chunfu Yang, Ming Li, John L K Mokili, Jorn Winter, Ndongala M. Lubaki, Kashamuka M. Mwandagalirwa, Mwamba J. Kasali, Atibu J. Losoma, Thomas C. Quinn, Robert C. Bollinger, Renu B. Lal

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

As the HIV-1 pandemic becomes increasingly complex, the genetic characterization of HIV strains bears important implications for vaccine research. To better understand the molecular evolution of HIV-1 viral diversity, we performed a comparative molecular analysis of HIV strains collected from high-risk persons in Kinshasa, Democratic Republic of Congo (DRC). Analysis of the gag-p24, env-C2V3 and -gp41 regions from 83 specimens collected in 1999-2000 revealed that 44 (53%) had concordant subtypes in the three regions (14 subsubtype A1, 10 subtype G, 8 subtype D, 5 subtype C, 2 each subsubtype F1 and CRF01_AE, and one each of subtypes H and J, and subsubtype A2, while the remaining 39 (47%) had mosaic genomes comprising multiple subtype combinations. Similar multisubtype patterns were also observed in 24 specimens collected in 1985. Sequence analysis of the gag-pol region (2.1 kb) from 21 discordant specimens in the gag-p24, env-C2V3 and -gp41 regions in 1985 and 1999-2000 further confirmed the complex recombinant patterns. Despite the remarkable similarity in overall subtype distribution, the intra- and intersubtype distances of major subtypes A1 and G increased significantly from 1985 to 1999-2000 (p = 0.018 and p = 0.0016, respectively). Given the complexity of HIV-1 viruses circulating in DRC, efforts should focus on the development of vaccines that result in cross-clade immunity.

Original languageEnglish (US)
Pages (from-to)661-666
Number of pages6
JournalAIDS Research and Human Retroviruses
Volume21
Issue number7
DOIs
StatePublished - Jul 2005
Externally publishedYes

Fingerprint

Democratic Republic of the Congo
Genetic Recombination
HIV-1
Vaccines
HIV
Molecular Evolution
Pandemics
varespladib methyl
Sequence Analysis
Immunity
Genome
Viruses
Research

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Yang, C., Li, M., Mokili, J. L. K., Winter, J., Lubaki, N. M., Mwandagalirwa, K. M., ... Lal, R. B. (2005). Genetic diversification and recombination of HIV type 1 group M in Kinshasa, Democratic Republic of Congo. AIDS Research and Human Retroviruses, 21(7), 661-666. https://doi.org/10.1089/aid.2005.21.661

Genetic diversification and recombination of HIV type 1 group M in Kinshasa, Democratic Republic of Congo. / Yang, Chunfu; Li, Ming; Mokili, John L K; Winter, Jorn; Lubaki, Ndongala M.; Mwandagalirwa, Kashamuka M.; Kasali, Mwamba J.; Losoma, Atibu J.; Quinn, Thomas C.; Bollinger, Robert C.; Lal, Renu B.

In: AIDS Research and Human Retroviruses, Vol. 21, No. 7, 07.2005, p. 661-666.

Research output: Contribution to journalArticle

Yang, C, Li, M, Mokili, JLK, Winter, J, Lubaki, NM, Mwandagalirwa, KM, Kasali, MJ, Losoma, AJ, Quinn, TC, Bollinger, RC & Lal, RB 2005, 'Genetic diversification and recombination of HIV type 1 group M in Kinshasa, Democratic Republic of Congo', AIDS Research and Human Retroviruses, vol. 21, no. 7, pp. 661-666. https://doi.org/10.1089/aid.2005.21.661
Yang, Chunfu ; Li, Ming ; Mokili, John L K ; Winter, Jorn ; Lubaki, Ndongala M. ; Mwandagalirwa, Kashamuka M. ; Kasali, Mwamba J. ; Losoma, Atibu J. ; Quinn, Thomas C. ; Bollinger, Robert C. ; Lal, Renu B. / Genetic diversification and recombination of HIV type 1 group M in Kinshasa, Democratic Republic of Congo. In: AIDS Research and Human Retroviruses. 2005 ; Vol. 21, No. 7. pp. 661-666.
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