Genetic diversity and recombination of enterovirus G strains in Japanese pigs: High prevalence of strains carrying a papain-like cysteine protease sequence in the enterovirus G population

Shinobu Tsuchiaka, Yuki Naoi, Ryo Imai, Tsuneyuki Masuda, Mika Ito, Masataka Akagami, Yoshinao Ouchi, Kazuo Ishii, Shoichi Sakaguchi, Tsutomu Omatsu, Yukie Katayama, Mami Oba, Junsuke Shirai, Yuki Satani, Yasuhiro Takashima, Yuji Taniguchi, Masaki Takasu, Hiroo Madarame, Fujiko Sunaga, Hiroshi AokiShinji Makino, Tetsuya Mizutani, Makoto Nagai

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

To study the genetic diversity of enterovirus G (EV-G) among Japanese pigs, metagenomics sequencing was performed on fecal samples from pigs with or without diarrhea, collected between 2014 and 2016. Fifty-nine EV-G sequences, which were >5,000 nucleotides long, were obtained. By complete VP1 sequence analysis, Japanese EV-G isolates were classified into G1 (17 strains), G2 (four strains), G3 (22 strains), G4 (two strains), G6 (two strains), G9 (six strains), G10 (five strains), and a new genotype (one strain). Remarkably, 16 G1 and one G2 strain identified in diarrheic (23.5%; four strains) or normal (76.5%; 13 strains) fecal samples possessed a papain-like cysteine protease (PL-CP) sequence, which was recently found in the USA and Belgium in the EV-G genome, at the 2C–3A junction site. This paper presents the first report of the high prevalence of viruses carrying PL-CP in the EV-G population. Furthermore, possible inter- and intragenotype recombination events were found among EV-G strains, including G1-PL-CP strains. Our findings may advance the understanding of the molecular epidemiology and genetic evolution of EV-Gs.

Original languageEnglish (US)
Article numbere0190819
JournalPLoS One
Volume13
Issue number1
DOIs
StatePublished - Jan 1 2018

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Enterovirus G
genetic recombination
Papain
Cysteine Proteases
papain
Enterovirus
cysteine proteinases
Genetic Recombination
Swine
genetic variation
swine
Population
Molecular Evolution
Viruses
Nucleotides
Genes
Metagenomics
Molecular Epidemiology
Belgium
Sequence Analysis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Genetic diversity and recombination of enterovirus G strains in Japanese pigs : High prevalence of strains carrying a papain-like cysteine protease sequence in the enterovirus G population. / Tsuchiaka, Shinobu; Naoi, Yuki; Imai, Ryo; Masuda, Tsuneyuki; Ito, Mika; Akagami, Masataka; Ouchi, Yoshinao; Ishii, Kazuo; Sakaguchi, Shoichi; Omatsu, Tsutomu; Katayama, Yukie; Oba, Mami; Shirai, Junsuke; Satani, Yuki; Takashima, Yasuhiro; Taniguchi, Yuji; Takasu, Masaki; Madarame, Hiroo; Sunaga, Fujiko; Aoki, Hiroshi; Makino, Shinji; Mizutani, Tetsuya; Nagai, Makoto.

In: PLoS One, Vol. 13, No. 1, e0190819, 01.01.2018.

Research output: Contribution to journalArticle

Tsuchiaka, S, Naoi, Y, Imai, R, Masuda, T, Ito, M, Akagami, M, Ouchi, Y, Ishii, K, Sakaguchi, S, Omatsu, T, Katayama, Y, Oba, M, Shirai, J, Satani, Y, Takashima, Y, Taniguchi, Y, Takasu, M, Madarame, H, Sunaga, F, Aoki, H, Makino, S, Mizutani, T & Nagai, M 2018, 'Genetic diversity and recombination of enterovirus G strains in Japanese pigs: High prevalence of strains carrying a papain-like cysteine protease sequence in the enterovirus G population', PLoS One, vol. 13, no. 1, e0190819. https://doi.org/10.1371/journal.pone.0190819
Tsuchiaka, Shinobu ; Naoi, Yuki ; Imai, Ryo ; Masuda, Tsuneyuki ; Ito, Mika ; Akagami, Masataka ; Ouchi, Yoshinao ; Ishii, Kazuo ; Sakaguchi, Shoichi ; Omatsu, Tsutomu ; Katayama, Yukie ; Oba, Mami ; Shirai, Junsuke ; Satani, Yuki ; Takashima, Yasuhiro ; Taniguchi, Yuji ; Takasu, Masaki ; Madarame, Hiroo ; Sunaga, Fujiko ; Aoki, Hiroshi ; Makino, Shinji ; Mizutani, Tetsuya ; Nagai, Makoto. / Genetic diversity and recombination of enterovirus G strains in Japanese pigs : High prevalence of strains carrying a papain-like cysteine protease sequence in the enterovirus G population. In: PLoS One. 2018 ; Vol. 13, No. 1.
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AU - Tsuchiaka, Shinobu

AU - Naoi, Yuki

AU - Imai, Ryo

AU - Masuda, Tsuneyuki

AU - Ito, Mika

AU - Akagami, Masataka

AU - Ouchi, Yoshinao

AU - Ishii, Kazuo

AU - Sakaguchi, Shoichi

AU - Omatsu, Tsutomu

AU - Katayama, Yukie

AU - Oba, Mami

AU - Shirai, Junsuke

AU - Satani, Yuki

AU - Takashima, Yasuhiro

AU - Taniguchi, Yuji

AU - Takasu, Masaki

AU - Madarame, Hiroo

AU - Sunaga, Fujiko

AU - Aoki, Hiroshi

AU - Makino, Shinji

AU - Mizutani, Tetsuya

AU - Nagai, Makoto

PY - 2018/1/1

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