TY - JOUR
T1 - Genetic diversity and recombination of enterovirus G strains in Japanese pigs
T2 - High prevalence of strains carrying a papain-like cysteine protease sequence in the enterovirus G population
AU - Tsuchiaka, Shinobu
AU - Naoi, Yuki
AU - Imai, Ryo
AU - Masuda, Tsuneyuki
AU - Ito, Mika
AU - Akagami, Masataka
AU - Ouchi, Yoshinao
AU - Ishii, Kazuo
AU - Sakaguchi, Shoichi
AU - Omatsu, Tsutomu
AU - Katayama, Yukie
AU - Oba, Mami
AU - Shirai, Junsuke
AU - Satani, Yuki
AU - Takashima, Yasuhiro
AU - Taniguchi, Yuji
AU - Takasu, Masaki
AU - Madarame, Hiroo
AU - Sunaga, Fujiko
AU - Aoki, Hiroshi
AU - Makino, Shinji
AU - Mizutani, Tetsuya
AU - Nagai, Makoto
N1 - Publisher Copyright:
© 2018 Tsuchiaka et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2018/1
Y1 - 2018/1
N2 - To study the genetic diversity of enterovirus G (EV-G) among Japanese pigs, metagenomics sequencing was performed on fecal samples from pigs with or without diarrhea, collected between 2014 and 2016. Fifty-nine EV-G sequences, which were >5,000 nucleotides long, were obtained. By complete VP1 sequence analysis, Japanese EV-G isolates were classified into G1 (17 strains), G2 (four strains), G3 (22 strains), G4 (two strains), G6 (two strains), G9 (six strains), G10 (five strains), and a new genotype (one strain). Remarkably, 16 G1 and one G2 strain identified in diarrheic (23.5%; four strains) or normal (76.5%; 13 strains) fecal samples possessed a papain-like cysteine protease (PL-CP) sequence, which was recently found in the USA and Belgium in the EV-G genome, at the 2C–3A junction site. This paper presents the first report of the high prevalence of viruses carrying PL-CP in the EV-G population. Furthermore, possible inter- and intragenotype recombination events were found among EV-G strains, including G1-PL-CP strains. Our findings may advance the understanding of the molecular epidemiology and genetic evolution of EV-Gs.
AB - To study the genetic diversity of enterovirus G (EV-G) among Japanese pigs, metagenomics sequencing was performed on fecal samples from pigs with or without diarrhea, collected between 2014 and 2016. Fifty-nine EV-G sequences, which were >5,000 nucleotides long, were obtained. By complete VP1 sequence analysis, Japanese EV-G isolates were classified into G1 (17 strains), G2 (four strains), G3 (22 strains), G4 (two strains), G6 (two strains), G9 (six strains), G10 (five strains), and a new genotype (one strain). Remarkably, 16 G1 and one G2 strain identified in diarrheic (23.5%; four strains) or normal (76.5%; 13 strains) fecal samples possessed a papain-like cysteine protease (PL-CP) sequence, which was recently found in the USA and Belgium in the EV-G genome, at the 2C–3A junction site. This paper presents the first report of the high prevalence of viruses carrying PL-CP in the EV-G population. Furthermore, possible inter- and intragenotype recombination events were found among EV-G strains, including G1-PL-CP strains. Our findings may advance the understanding of the molecular epidemiology and genetic evolution of EV-Gs.
UR - https://www.scopus.com/pages/publications/85040359141
UR - https://www.scopus.com/pages/publications/85040359141#tab=citedBy
U2 - 10.1371/journal.pone.0190819
DO - 10.1371/journal.pone.0190819
M3 - Article
C2 - 29324778
AN - SCOPUS:85040359141
SN - 1932-6203
VL - 13
JO - PloS one
JF - PloS one
IS - 1
M1 - e0190819
ER -