Genetic evidence of hantavirus infections in wild rodents from Northwestern Colombia

Andres F. Londoño, Francisco J. Díaz, Piedad Agudelo-Flórez, Silvana Levis, Juan D. Rodas

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

This report builds on recent serological evidence for the presence of hantavirus in northern Colombia by providing sequence-specific and phylogenetic data of hantavirus infections in wild rodents. From August 2007 to August 2008, 354 rodent specimens representing four families were collected in the northwestern Antioquia region of Colombia. Antibodies reactive to Sin Nombre virus and Maciel virus antigens by IgG enzyme-linked immunosorbent assay were found in 15 of 109 (14%) Cherries cane rats (Zygodontomys cherriei), the only sigmodontinae rodents captured. Lung tissue samples from 11 of the 15 seropositive rodents were RT-polymerase chain reaction positive for hantavirus RNA, using primers for the S and M genome segments. Eight of these amplicons were sequenced and phylogenetic analyses indicated RNA of a hantavirus closely related to Calabazo virus, previously found in Panama. This is the first report of the genetic characterization of a hantavirus in rodents in Colombia.

Original languageEnglish (US)
Pages (from-to)701-708
Number of pages8
JournalVector-Borne and Zoonotic Diseases
Volume11
Issue number6
DOIs
StatePublished - Jun 1 2011
Externally publishedYes

Fingerprint

Hantavirus Infections
Colombia
Hantavirus
Rodentia
Sin Nombre virus
Sigmodontinae
Viruses
Panama
Canes
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Genome
RNA
Antigens
Polymerase Chain Reaction
Lung
Antibodies

Keywords

  • Colombia
  • Hantavirus
  • Molecular phylogenetics
  • Reservoirs
  • Sequences
  • Wild rodents

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases
  • Virology

Cite this

Londoño, A. F., Díaz, F. J., Agudelo-Flórez, P., Levis, S., & Rodas, J. D. (2011). Genetic evidence of hantavirus infections in wild rodents from Northwestern Colombia. Vector-Borne and Zoonotic Diseases, 11(6), 701-708. https://doi.org/10.1089/vbz.2010.0129

Genetic evidence of hantavirus infections in wild rodents from Northwestern Colombia. / Londoño, Andres F.; Díaz, Francisco J.; Agudelo-Flórez, Piedad; Levis, Silvana; Rodas, Juan D.

In: Vector-Borne and Zoonotic Diseases, Vol. 11, No. 6, 01.06.2011, p. 701-708.

Research output: Contribution to journalArticle

Londoño, AF, Díaz, FJ, Agudelo-Flórez, P, Levis, S & Rodas, JD 2011, 'Genetic evidence of hantavirus infections in wild rodents from Northwestern Colombia', Vector-Borne and Zoonotic Diseases, vol. 11, no. 6, pp. 701-708. https://doi.org/10.1089/vbz.2010.0129
Londoño, Andres F. ; Díaz, Francisco J. ; Agudelo-Flórez, Piedad ; Levis, Silvana ; Rodas, Juan D. / Genetic evidence of hantavirus infections in wild rodents from Northwestern Colombia. In: Vector-Borne and Zoonotic Diseases. 2011 ; Vol. 11, No. 6. pp. 701-708.
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