Genetic expression profile during acute cellular rejection in clinical intestinal transplantation

Sean P. Bradley, Barbara Banner, George Elias, Mohan P. Pahari, Melissa Brown, Cristiana Rastellini, Luca Cicalese

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Small bowel transplantation is a successful treatment for irreversible intestinal failure. Acute cellular rejection (ACR) represents the major cause of graft loss. However, little is known regarding the mechanisms of ACR and no data exist on the genetic response occurring during ACR. We report a genetic expression profile determined using oligo-microarrays of the intestinal graft during an ACR episode. Mucosal biopsies were obtained from the graft at weeks 0, 1, 2, 3, 6, and 52 posttransplant. We observed a statistically significant increase in transcript levels from 51 genes between biopsy samples from before ACR compared with during ACR. Functional analysis of these genes revealed an interferon (IFN)-α signature associated with a type I IFN immune response from dendritic cells or association with cellular proliferation and division. These genetic data support that dendritic cell activation was ongoing during ACR and suggest that IFNα production as a potential immunosuppressive target for transplantation.

Original languageEnglish (US)
Pages (from-to)998-1001
Number of pages4
JournalTransplantation
Volume86
Issue number7
DOIs
StatePublished - Oct 15 2008

Fingerprint

Transplantation
Transplants
Dendritic Cells
Interferons
Biopsy
Interferon Type I
Immunosuppressive Agents
Genes
Cell Proliferation

Keywords

  • Acute cellular rejection
  • Dendritic cells
  • Gene expression
  • Intestinal transplant
  • Type I interferon

ASJC Scopus subject areas

  • Transplantation

Cite this

Genetic expression profile during acute cellular rejection in clinical intestinal transplantation. / Bradley, Sean P.; Banner, Barbara; Elias, George; Pahari, Mohan P.; Brown, Melissa; Rastellini, Cristiana; Cicalese, Luca.

In: Transplantation, Vol. 86, No. 7, 15.10.2008, p. 998-1001.

Research output: Contribution to journalArticle

Bradley, Sean P. ; Banner, Barbara ; Elias, George ; Pahari, Mohan P. ; Brown, Melissa ; Rastellini, Cristiana ; Cicalese, Luca. / Genetic expression profile during acute cellular rejection in clinical intestinal transplantation. In: Transplantation. 2008 ; Vol. 86, No. 7. pp. 998-1001.
@article{fd65e68e8f8740df8358c1b195f98f5d,
title = "Genetic expression profile during acute cellular rejection in clinical intestinal transplantation",
abstract = "Small bowel transplantation is a successful treatment for irreversible intestinal failure. Acute cellular rejection (ACR) represents the major cause of graft loss. However, little is known regarding the mechanisms of ACR and no data exist on the genetic response occurring during ACR. We report a genetic expression profile determined using oligo-microarrays of the intestinal graft during an ACR episode. Mucosal biopsies were obtained from the graft at weeks 0, 1, 2, 3, 6, and 52 posttransplant. We observed a statistically significant increase in transcript levels from 51 genes between biopsy samples from before ACR compared with during ACR. Functional analysis of these genes revealed an interferon (IFN)-α signature associated with a type I IFN immune response from dendritic cells or association with cellular proliferation and division. These genetic data support that dendritic cell activation was ongoing during ACR and suggest that IFNα production as a potential immunosuppressive target for transplantation.",
keywords = "Acute cellular rejection, Dendritic cells, Gene expression, Intestinal transplant, Type I interferon",
author = "Bradley, {Sean P.} and Barbara Banner and George Elias and Pahari, {Mohan P.} and Melissa Brown and Cristiana Rastellini and Luca Cicalese",
year = "2008",
month = "10",
day = "15",
doi = "10.1097/TP.0b013e3181874989",
language = "English (US)",
volume = "86",
pages = "998--1001",
journal = "Transplantation",
issn = "0041-1337",
publisher = "Lippincott Williams and Wilkins",
number = "7",

}

TY - JOUR

T1 - Genetic expression profile during acute cellular rejection in clinical intestinal transplantation

AU - Bradley, Sean P.

AU - Banner, Barbara

AU - Elias, George

AU - Pahari, Mohan P.

AU - Brown, Melissa

AU - Rastellini, Cristiana

AU - Cicalese, Luca

PY - 2008/10/15

Y1 - 2008/10/15

N2 - Small bowel transplantation is a successful treatment for irreversible intestinal failure. Acute cellular rejection (ACR) represents the major cause of graft loss. However, little is known regarding the mechanisms of ACR and no data exist on the genetic response occurring during ACR. We report a genetic expression profile determined using oligo-microarrays of the intestinal graft during an ACR episode. Mucosal biopsies were obtained from the graft at weeks 0, 1, 2, 3, 6, and 52 posttransplant. We observed a statistically significant increase in transcript levels from 51 genes between biopsy samples from before ACR compared with during ACR. Functional analysis of these genes revealed an interferon (IFN)-α signature associated with a type I IFN immune response from dendritic cells or association with cellular proliferation and division. These genetic data support that dendritic cell activation was ongoing during ACR and suggest that IFNα production as a potential immunosuppressive target for transplantation.

AB - Small bowel transplantation is a successful treatment for irreversible intestinal failure. Acute cellular rejection (ACR) represents the major cause of graft loss. However, little is known regarding the mechanisms of ACR and no data exist on the genetic response occurring during ACR. We report a genetic expression profile determined using oligo-microarrays of the intestinal graft during an ACR episode. Mucosal biopsies were obtained from the graft at weeks 0, 1, 2, 3, 6, and 52 posttransplant. We observed a statistically significant increase in transcript levels from 51 genes between biopsy samples from before ACR compared with during ACR. Functional analysis of these genes revealed an interferon (IFN)-α signature associated with a type I IFN immune response from dendritic cells or association with cellular proliferation and division. These genetic data support that dendritic cell activation was ongoing during ACR and suggest that IFNα production as a potential immunosuppressive target for transplantation.

KW - Acute cellular rejection

KW - Dendritic cells

KW - Gene expression

KW - Intestinal transplant

KW - Type I interferon

UR - http://www.scopus.com/inward/record.url?scp=56049088505&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=56049088505&partnerID=8YFLogxK

U2 - 10.1097/TP.0b013e3181874989

DO - 10.1097/TP.0b013e3181874989

M3 - Article

VL - 86

SP - 998

EP - 1001

JO - Transplantation

JF - Transplantation

SN - 0041-1337

IS - 7

ER -