Genetic incorporation of an aliphatic keto-containing amino acid into proteins for their site-specific modifications

Ying Huang, Wei Wan, William K. Russell, Pei Jing Pai, Zhiyong Wang, David H. Russell, Wenshe Liu

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

2-Amino-8-oxononanoic acid has been genetically incorporated into proteins in Escherichia coli by the use of an evolved pyrrolysyl-tRNA synthetase/pylT pair. The direct usage of the exclusive reactivity of the keto group in this amino acid with hydrazide- and alkoxyamine-bearing compounds to site-specifically label proteins under a mild condition close to physiological pH exhibited a very high efficiency.

Original languageEnglish (US)
Pages (from-to)878-880
Number of pages3
JournalBioorganic and Medicinal Chemistry Letters
Volume20
Issue number3
DOIs
StatePublished - Feb 1 2010
Externally publishedYes

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Keywords

  • 2-Amino-8-oxononanoic acid
  • Amber suppression
  • Genetic code expansion
  • Keto
  • Ne-acetyl-lysine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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