Genetic loci of Mycoplasma agalactiae involved in systemic spreading during experimental intramammary infection of sheep

Shivanand Hegde, Martina Zimmermann, Martina Flöck, Rene Brunthaler, Joachim Spergser, Renate Rosengarten, Rohini Chopra-Dewasthaly

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Mycoplasmas are amongst the most successful pathogens of both humans and animals yet the molecular basis of mycoplasma pathogenesis is poorly understood. This is partly due to the lack of classical virulence factors and little similarity to common bacterial pathogenic determinants. Using Mycoplasma agalactiae as a model we initiated research in this direction by screening a transposon mutant library in the natural sheep host using a negative selection method. Having successfully identified putative factors involved in the colonization of local infection and lymphogenic sites, the current study assessed mutants unable to spread systemically in sheep after experimental intramammary infection. Analysis of distant body sites for complete absence of mutants via SSM PCR revealed that additional set of genes, such as pdhB, oppC, oppB, gtsB, MAG1890, MAG5520 and MAG3650 are required for systemic spreading apart from those that were necessary for initial colonization. Additional in vitro studies with the mutants absent at these systemic sites confirmed the potential role of some of the respective gene products concerning their interaction with host cells. Mutants of pdhB, oppC and MAG4460 exhibited significantly slower growth in the presence of HeLa cells in MEM medium. This first attempt to identify genes exclusively required for systemic spreading provides a basis for further in-depth research to understand the exact mechanism of chronicity and persistence of M. agalactiae.

Original languageEnglish (US)
Article number106
JournalVeterinary Research
Volume47
Issue number1
DOIs
StatePublished - Oct 20 2016

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Mycoplasma agalactiae
Genetic Loci
Sheep
Mycoplasma
sheep
mutants
loci
Infection
infection
Genes
Virulence Factors
HeLa Cells
Research
genes
Polymerase Chain Reaction
selection methods
transposons
in vitro studies
Growth
virulence

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Hegde, S., Zimmermann, M., Flöck, M., Brunthaler, R., Spergser, J., Rosengarten, R., & Chopra-Dewasthaly, R. (2016). Genetic loci of Mycoplasma agalactiae involved in systemic spreading during experimental intramammary infection of sheep. Veterinary Research, 47(1), [106]. https://doi.org/10.1186/s13567-016-0387-0

Genetic loci of Mycoplasma agalactiae involved in systemic spreading during experimental intramammary infection of sheep. / Hegde, Shivanand; Zimmermann, Martina; Flöck, Martina; Brunthaler, Rene; Spergser, Joachim; Rosengarten, Renate; Chopra-Dewasthaly, Rohini.

In: Veterinary Research, Vol. 47, No. 1, 106, 20.10.2016.

Research output: Contribution to journalArticle

Hegde, S, Zimmermann, M, Flöck, M, Brunthaler, R, Spergser, J, Rosengarten, R & Chopra-Dewasthaly, R 2016, 'Genetic loci of Mycoplasma agalactiae involved in systemic spreading during experimental intramammary infection of sheep', Veterinary Research, vol. 47, no. 1, 106. https://doi.org/10.1186/s13567-016-0387-0
Hegde, Shivanand ; Zimmermann, Martina ; Flöck, Martina ; Brunthaler, Rene ; Spergser, Joachim ; Rosengarten, Renate ; Chopra-Dewasthaly, Rohini. / Genetic loci of Mycoplasma agalactiae involved in systemic spreading during experimental intramammary infection of sheep. In: Veterinary Research. 2016 ; Vol. 47, No. 1.
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