Genetic transformation and phylogeny of bacterial symbionts from tsetse.

C. B. Beard, S. L. O'Neill, P. Mason, L. Mandelco, C. R. Woese, R. B. Tesh, F. F. Richards, S. Aksoy

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Two isolates of bacterial endosymbionts, GP01 and GM02, were established in cell free medium from haemolymph of the tsetse, Glossina pallidipes and G. morsitans. These microorganisms appear similar to rickettsia-like organisms reported previously from various tsetse species. The 16S rRNA sequence analysis, however, placed them within the gamma subdivision of the Proteobacteria, phylogenetically distinct from most members of the Rickettsiaceae which align with the alpha subdivision. Distinct multiple endogenous plasmids are harboured by GP01 and GM02, suggesting that the two isolates are different. Restriction mapping analysis showed that one of the conserved plasmids is present in high copy number and is at least 80 kb in size. A heterologous plasmid pSUP204, which contains the broad host range oriV replication origin, was used to transfect bacterial cultures. The symbiont GM02 was transformed, and it expressed plasmid encoded resistance to the antibiotics ampicillin, tetracycline and chloramphenicol. Transformation of these symbionts may provide a novel means for expressing anti-parasitic genes within tsetse populations.

Original languageEnglish (US)
Pages (from-to)123-131
Number of pages9
JournalInsect Molecular Biology
Volume1
Issue number3
StatePublished - 1993
Externally publishedYes

Fingerprint

Genetic Transformation
Glossina
genetic transformation
Phylogeny
symbionts
plasmids
Plasmids
phylogeny
Rickettsiaceae
rickettsia-like organisms
Glossina morsitans
Glossina pallidipes
Tsetse Flies
Gammaproteobacteria
replication origin
Rickettsia
Restriction Mapping
Replication Origin
restriction mapping
Hemolymph

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Beard, C. B., O'Neill, S. L., Mason, P., Mandelco, L., Woese, C. R., Tesh, R. B., ... Aksoy, S. (1993). Genetic transformation and phylogeny of bacterial symbionts from tsetse. Insect Molecular Biology, 1(3), 123-131.

Genetic transformation and phylogeny of bacterial symbionts from tsetse. / Beard, C. B.; O'Neill, S. L.; Mason, P.; Mandelco, L.; Woese, C. R.; Tesh, R. B.; Richards, F. F.; Aksoy, S.

In: Insect Molecular Biology, Vol. 1, No. 3, 1993, p. 123-131.

Research output: Contribution to journalArticle

Beard, CB, O'Neill, SL, Mason, P, Mandelco, L, Woese, CR, Tesh, RB, Richards, FF & Aksoy, S 1993, 'Genetic transformation and phylogeny of bacterial symbionts from tsetse.', Insect Molecular Biology, vol. 1, no. 3, pp. 123-131.
Beard CB, O'Neill SL, Mason P, Mandelco L, Woese CR, Tesh RB et al. Genetic transformation and phylogeny of bacterial symbionts from tsetse. Insect Molecular Biology. 1993;1(3):123-131.
Beard, C. B. ; O'Neill, S. L. ; Mason, P. ; Mandelco, L. ; Woese, C. R. ; Tesh, R. B. ; Richards, F. F. ; Aksoy, S. / Genetic transformation and phylogeny of bacterial symbionts from tsetse. In: Insect Molecular Biology. 1993 ; Vol. 1, No. 3. pp. 123-131.
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