Genetic transformation of cultivated jute (Corchorus capsularis L.) by particle bombardment using apical meristem tissue and development of stable transgenic plant

Jagannath Bhattacharyya, Anirban Chakraborty, Souri Roy, Subrata Pradhan, Joy Mitra, Monami Chakraborty, Anulina Manna, Narattam Sikdar, Saikat Chakraborty, Soumitra Kumar Sen

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

An in vitro plant regeneration and genetic transformation protocol was established in jute (Corchorus capsularis L. var JRC321). One-day-old apical, meristematic tissues of germinating seedlings were used as explants. Multiple shoots were regenerated from each explant using Murashige and Skoog basal medium containing 1.78 µM benzylamino purine and 4.92 µM indole-3-butyric acid. Transformation was carried out in three independent sets (each set comprising of three independent experiments each comprising three replications with 35 explants per replication) using the bialaphos resistance gene (bar), synthetically designed for high level plant expression. The positive transformants containing the bar gene were selected in growth medium containing 2.5 mg/l bialaphos. Polymerase chain reaction (PCR), Southern and northern blots, real-time quantitative PCR, western blot and enzymatic assay of five putative transformants from three independent sets provided evidence for full-length gene integration into the genomic DNA of transformed jute, as well as high level expression of the transgene. Analysis of the T1 plants revealed a stable inheritance of the transgene through the progenies. The data presented in this report showed considerable advancement in jute transformation and should improve future genetic engineering strategies to be employed for improvement of this very important fibre crop.

Original languageEnglish (US)
Pages (from-to)311-324
Number of pages14
JournalPlant Cell, Tissue and Organ Culture
Volume121
Issue number2
DOIs
StatePublished - May 1 2015
Externally publishedYes

Keywords

  • Bar gene
  • Bialaphos
  • Herbicide resistance
  • Jute transformation
  • Particle bombardment
  • Transgenic plants

ASJC Scopus subject areas

  • Horticulture

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