Genistein treatment of cells inhibits arenavirus infection

Eric M. Vela, Gavin C. Bowick, Norbert K. Herzog, Judith Aronson

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Arenaviridae is a family of enveloped viruses some of which are capable of causing hemorrhagic fever syndromes in humans. In this report, we demonstrate that treatment of host cells with the tyrosine kinase inhibitor genistein inhibits infection of cells with the New World arenavirus Pichindé (PICV). The greatest degree of inhibition was observed in pre-treated target cells, but modest inhibition of infection was also seen when drug was added to cultures up to 48 h after infection. We show that PICV-induced phosphorylation of the activating transcription factor-2 protein (ATF-2) and cyclic adenosine monophosphate response element binding protein (CREB) is inhibited following genistein treatment. Lastly, genistein treatment also inhibited transduction of cells with pseudotyped retrovirus particles expressing envelope proteins of the Old World arenavirus Lassa virus. These results demonstrate that kinase activity is required for arenavirus infection and that therapeutics designed to inhibit kinase activity should be explored.

Original languageEnglish (US)
Pages (from-to)153-156
Number of pages4
JournalAntiviral Research
Volume77
Issue number2
DOIs
StatePublished - Feb 2008
Externally publishedYes

Fingerprint

Arenaviridae Infections
Genistein
Old World Arenaviruses
Arenaviridae
New World Arenaviruses
Phosphotransferases
Activating Transcription Factor 2
Lassa virus
Infection
CREB-Binding Protein
Response Elements
Retroviridae
Adenosine Monophosphate
Therapeutics
Protein-Tyrosine Kinases
Proteins
Fever
Phosphorylation
Viruses
Pharmaceutical Preparations

Keywords

  • Arenavirus
  • ATF-2
  • CREB
  • Genistein
  • Lassa virus
  • Pichindé virus

ASJC Scopus subject areas

  • Virology
  • Pharmacology

Cite this

Genistein treatment of cells inhibits arenavirus infection. / Vela, Eric M.; Bowick, Gavin C.; Herzog, Norbert K.; Aronson, Judith.

In: Antiviral Research, Vol. 77, No. 2, 02.2008, p. 153-156.

Research output: Contribution to journalArticle

Vela, Eric M. ; Bowick, Gavin C. ; Herzog, Norbert K. ; Aronson, Judith. / Genistein treatment of cells inhibits arenavirus infection. In: Antiviral Research. 2008 ; Vol. 77, No. 2. pp. 153-156.
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