Genome anaylsis and phylogenetic relationships between east, central and west African isolates of Yellow fever virus

Jana J. von Lindern, Sarah Aroner, Nicolas D. Barrett, Jason A. Wicker, C. Todd Davis, Alan Barrett

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Yellow fever virus (YFV), a reemerging disease agent in Africa and South America, is the prototype member of the genus Flavivirus. Based on examination of the prM/M, E and 3′ non-coding regions of the YFV genome, previous studies have identified seven genotypes of YFV, including the Angolan, east/central African and east African genotypes, which are highly divergent from the prototype strain Asibi. In this study, full genome analysis was used to expand upon these genetic relationships as well as on the very limited full genome database for YFV. This study was the first to investigate genomic sequences of YFV strains from east and central Africa (Angola71, Uganda48a and Ethiopia61b). All three viruses had genomes of 10 823 nt in length. Compared with the prototype strain Asibi (from west Africa) they were approximately 25 % divergent in nucleotide sequence and 7 % divergent in amino acid sequence. Comparison of multiple flaviviruses in the N-terminal region of NS4B showed that amino acid sequences were variable and that west African strains of YFV had an amino acid deletion at residue 21. Additionally, N-linked glycosylation sites were conserved between viral genotypes, while codon usage varied between strains.

Original languageEnglish (US)
Pages (from-to)895-907
Number of pages13
JournalJournal of General Virology
Volume87
Issue number4
DOIs
StatePublished - Apr 2006

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Yellow fever virus
Genome
Flavivirus
Genotype
Amino Acid Sequence
Central Africa
Eastern Africa
Western Africa
South America
Glycosylation
Codon
Databases
Viruses
Amino Acids

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Genome anaylsis and phylogenetic relationships between east, central and west African isolates of Yellow fever virus. / von Lindern, Jana J.; Aroner, Sarah; Barrett, Nicolas D.; Wicker, Jason A.; Davis, C. Todd; Barrett, Alan.

In: Journal of General Virology, Vol. 87, No. 4, 04.2006, p. 895-907.

Research output: Contribution to journalArticle

von Lindern, Jana J. ; Aroner, Sarah ; Barrett, Nicolas D. ; Wicker, Jason A. ; Davis, C. Todd ; Barrett, Alan. / Genome anaylsis and phylogenetic relationships between east, central and west African isolates of Yellow fever virus. In: Journal of General Virology. 2006 ; Vol. 87, No. 4. pp. 895-907.
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