Genome sequence diversity and clues to the evolution of variola (smallpox) virus

Joseph J. Esposito, Scott A. Sammons, A. Michael Frace, John D. Osborne, Melissa Glsen-Rasmussen, Ming Zhang, Dhwani Govil, Inger K. Damon, Richard Kline, Miriam Laker, Yu Li, Geoffrey L. Smith, Hermann Meyer, James LeDuc, Robert M. Wohlhueter

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

Comparative genomics of 45 epidemiologically varied variola virus isolates from the past 30 years of the smallpox era indicate low sequence diversity, suggesting that there is probably little difference in the isolates' functional gene content. Phylogenetic clustering inferred three clades coincident with their geographical origin and case-fatality rate; the latter implicated putative proteins that mediate viral virulence differences. Analysis of the viral linear DNA genome suggests that its evolution involved direct descent and DNA end-region recombination events. Knowing the sequences will help understand the viral proteome and improve diagnostic test precision, therapeutics, and systems for their assessment.

Original languageEnglish (US)
Pages (from-to)807-812
Number of pages6
JournalScience
Volume313
Issue number5788
DOIs
StatePublished - Aug 11 2006
Externally publishedYes

Fingerprint

Variola virus
Smallpox
Viral DNA
Viral Proteins
Proteome
Genomics
Routine Diagnostic Tests
Genetic Recombination
Cluster Analysis
Virulence
Genome
Mortality
DNA
Genes
Therapeutics

ASJC Scopus subject areas

  • General

Cite this

Esposito, J. J., Sammons, S. A., Frace, A. M., Osborne, J. D., Glsen-Rasmussen, M., Zhang, M., ... Wohlhueter, R. M. (2006). Genome sequence diversity and clues to the evolution of variola (smallpox) virus. Science, 313(5788), 807-812. https://doi.org/10.1126/science.1125134

Genome sequence diversity and clues to the evolution of variola (smallpox) virus. / Esposito, Joseph J.; Sammons, Scott A.; Frace, A. Michael; Osborne, John D.; Glsen-Rasmussen, Melissa; Zhang, Ming; Govil, Dhwani; Damon, Inger K.; Kline, Richard; Laker, Miriam; Li, Yu; Smith, Geoffrey L.; Meyer, Hermann; LeDuc, James; Wohlhueter, Robert M.

In: Science, Vol. 313, No. 5788, 11.08.2006, p. 807-812.

Research output: Contribution to journalArticle

Esposito, JJ, Sammons, SA, Frace, AM, Osborne, JD, Glsen-Rasmussen, M, Zhang, M, Govil, D, Damon, IK, Kline, R, Laker, M, Li, Y, Smith, GL, Meyer, H, LeDuc, J & Wohlhueter, RM 2006, 'Genome sequence diversity and clues to the evolution of variola (smallpox) virus', Science, vol. 313, no. 5788, pp. 807-812. https://doi.org/10.1126/science.1125134
Esposito JJ, Sammons SA, Frace AM, Osborne JD, Glsen-Rasmussen M, Zhang M et al. Genome sequence diversity and clues to the evolution of variola (smallpox) virus. Science. 2006 Aug 11;313(5788):807-812. https://doi.org/10.1126/science.1125134
Esposito, Joseph J. ; Sammons, Scott A. ; Frace, A. Michael ; Osborne, John D. ; Glsen-Rasmussen, Melissa ; Zhang, Ming ; Govil, Dhwani ; Damon, Inger K. ; Kline, Richard ; Laker, Miriam ; Li, Yu ; Smith, Geoffrey L. ; Meyer, Hermann ; LeDuc, James ; Wohlhueter, Robert M. / Genome sequence diversity and clues to the evolution of variola (smallpox) virus. In: Science. 2006 ; Vol. 313, No. 5788. pp. 807-812.
@article{812dcc585eb5420bbb5c5359d7045d5f,
title = "Genome sequence diversity and clues to the evolution of variola (smallpox) virus",
abstract = "Comparative genomics of 45 epidemiologically varied variola virus isolates from the past 30 years of the smallpox era indicate low sequence diversity, suggesting that there is probably little difference in the isolates' functional gene content. Phylogenetic clustering inferred three clades coincident with their geographical origin and case-fatality rate; the latter implicated putative proteins that mediate viral virulence differences. Analysis of the viral linear DNA genome suggests that its evolution involved direct descent and DNA end-region recombination events. Knowing the sequences will help understand the viral proteome and improve diagnostic test precision, therapeutics, and systems for their assessment.",
author = "Esposito, {Joseph J.} and Sammons, {Scott A.} and Frace, {A. Michael} and Osborne, {John D.} and Melissa Glsen-Rasmussen and Ming Zhang and Dhwani Govil and Damon, {Inger K.} and Richard Kline and Miriam Laker and Yu Li and Smith, {Geoffrey L.} and Hermann Meyer and James LeDuc and Wohlhueter, {Robert M.}",
year = "2006",
month = "8",
day = "11",
doi = "10.1126/science.1125134",
language = "English (US)",
volume = "313",
pages = "807--812",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5788",

}

TY - JOUR

T1 - Genome sequence diversity and clues to the evolution of variola (smallpox) virus

AU - Esposito, Joseph J.

AU - Sammons, Scott A.

AU - Frace, A. Michael

AU - Osborne, John D.

AU - Glsen-Rasmussen, Melissa

AU - Zhang, Ming

AU - Govil, Dhwani

AU - Damon, Inger K.

AU - Kline, Richard

AU - Laker, Miriam

AU - Li, Yu

AU - Smith, Geoffrey L.

AU - Meyer, Hermann

AU - LeDuc, James

AU - Wohlhueter, Robert M.

PY - 2006/8/11

Y1 - 2006/8/11

N2 - Comparative genomics of 45 epidemiologically varied variola virus isolates from the past 30 years of the smallpox era indicate low sequence diversity, suggesting that there is probably little difference in the isolates' functional gene content. Phylogenetic clustering inferred three clades coincident with their geographical origin and case-fatality rate; the latter implicated putative proteins that mediate viral virulence differences. Analysis of the viral linear DNA genome suggests that its evolution involved direct descent and DNA end-region recombination events. Knowing the sequences will help understand the viral proteome and improve diagnostic test precision, therapeutics, and systems for their assessment.

AB - Comparative genomics of 45 epidemiologically varied variola virus isolates from the past 30 years of the smallpox era indicate low sequence diversity, suggesting that there is probably little difference in the isolates' functional gene content. Phylogenetic clustering inferred three clades coincident with their geographical origin and case-fatality rate; the latter implicated putative proteins that mediate viral virulence differences. Analysis of the viral linear DNA genome suggests that its evolution involved direct descent and DNA end-region recombination events. Knowing the sequences will help understand the viral proteome and improve diagnostic test precision, therapeutics, and systems for their assessment.

UR - http://www.scopus.com/inward/record.url?scp=33747106195&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747106195&partnerID=8YFLogxK

U2 - 10.1126/science.1125134

DO - 10.1126/science.1125134

M3 - Article

C2 - 16873609

AN - SCOPUS:33747106195

VL - 313

SP - 807

EP - 812

JO - Science

JF - Science

SN - 0036-8075

IS - 5788

ER -