Genome-wide mapping of long-range contacts unveils clustering of DNA double-strand breaks at damaged active genes

François Aymard, Marion Aguirrebengoa, Emmanuelle Guillou, Biola M. Javierre, Beatrix Bugler, Coline Arnould, Vincent Rocher, Jason S. Iacovoni, Anna Biernacka, Magdalena Skrzypczak, Krzysztof Ginalski, Malgorzata Rowicka-Kudlicka, Peter Fraser, Gaëlle Legube

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The ability of DNA double-strand breaks (DSBs) to cluster in mammalian cells has been a subject of intense debate in recent years. Here we used a high-throughput chromosome conformation capture assay (capture Hi-C) to investigate clustering of DSBs induced at defined loci in the human genome. The results unambiguously demonstrated that DSBs cluster, but only when they are induced within transcriptionally active genes. Clustering of damaged genes occurs primarily during the G1 cell-cycle phase and coincides with delayed repair. Moreover, DSB clustering depends on the MRN complex as well as the Formin 2 (FMN2) nuclear actin organizer and the linker of nuclear and cytoplasmic skeleton (LINC) complex, thus suggesting that active mechanisms promote clustering. This work reveals that, when damaged, active genes, compared with the rest of the genome, exhibit a distinctive behavior, remaining largely unrepaired and clustered in G1, and being repaired via homologous recombination in postreplicative cells.

Original languageEnglish (US)
Pages (from-to)353-361
Number of pages9
JournalNature Structural and Molecular Biology
Volume24
Issue number4
DOIs
StatePublished - Apr 1 2017

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Double-Stranded DNA Breaks
Chromosome Mapping
Cluster Analysis
Genes
Homologous Recombination
Human Genome
Skeleton
Actins
Cell Cycle
Chromosomes
Genome

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Aymard, F., Aguirrebengoa, M., Guillou, E., Javierre, B. M., Bugler, B., Arnould, C., ... Legube, G. (2017). Genome-wide mapping of long-range contacts unveils clustering of DNA double-strand breaks at damaged active genes. Nature Structural and Molecular Biology, 24(4), 353-361. https://doi.org/10.1038/nsmb.3387

Genome-wide mapping of long-range contacts unveils clustering of DNA double-strand breaks at damaged active genes. / Aymard, François; Aguirrebengoa, Marion; Guillou, Emmanuelle; Javierre, Biola M.; Bugler, Beatrix; Arnould, Coline; Rocher, Vincent; Iacovoni, Jason S.; Biernacka, Anna; Skrzypczak, Magdalena; Ginalski, Krzysztof; Rowicka-Kudlicka, Malgorzata; Fraser, Peter; Legube, Gaëlle.

In: Nature Structural and Molecular Biology, Vol. 24, No. 4, 01.04.2017, p. 353-361.

Research output: Contribution to journalArticle

Aymard, F, Aguirrebengoa, M, Guillou, E, Javierre, BM, Bugler, B, Arnould, C, Rocher, V, Iacovoni, JS, Biernacka, A, Skrzypczak, M, Ginalski, K, Rowicka-Kudlicka, M, Fraser, P & Legube, G 2017, 'Genome-wide mapping of long-range contacts unveils clustering of DNA double-strand breaks at damaged active genes', Nature Structural and Molecular Biology, vol. 24, no. 4, pp. 353-361. https://doi.org/10.1038/nsmb.3387
Aymard, François ; Aguirrebengoa, Marion ; Guillou, Emmanuelle ; Javierre, Biola M. ; Bugler, Beatrix ; Arnould, Coline ; Rocher, Vincent ; Iacovoni, Jason S. ; Biernacka, Anna ; Skrzypczak, Magdalena ; Ginalski, Krzysztof ; Rowicka-Kudlicka, Malgorzata ; Fraser, Peter ; Legube, Gaëlle. / Genome-wide mapping of long-range contacts unveils clustering of DNA double-strand breaks at damaged active genes. In: Nature Structural and Molecular Biology. 2017 ; Vol. 24, No. 4. pp. 353-361.
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