TY - JOUR
T1 - Genome-wide tracing to decipher nuclear organization
AU - Flores, Victoria
AU - Farabella, Irene
AU - Nir, Guy
N1 - Funding Information:
We would like to thank Laura Taverna, from the Outreach and Digital Production Office at IIIT, for her help with the figure. This work was funded by UTMB start-up funds and Cancer Prevention & Research Institute of Texas (CPRIT) grant ID RR210018 to G.N. and by The Giovanni Armenise-Harvard Foundation to I.F. (Career Development Award, 2022).
Publisher Copyright:
© 2023 Elsevier Ltd
PY - 2023/6
Y1 - 2023/6
N2 - Nuclear organization impacts gene expression activity and cell phenotype. Our current understanding is mainly derived from ensemble-level sequencing studies that reflect the 3D genome structure of millions of cells. These approaches have provided invaluable details on the 3D organizations of the genome and their relation to other nuclear landmarks. However, they mostly lack the ability to provide multimodal information simultaneously at the single-cell level. In recent years, cutting-edge imaging technologies have risen to the challenge of simultaneously describing multiple components of the nuclear space at the single-cell level, paving the way for a deeper understanding of the genome structure–function relationship. This review will focus on the development and utilization of such technologies to gain a multi-component view of the nucleus at single-cell resolution, dissecting the complexity and heterogeneity of nuclear organization.
AB - Nuclear organization impacts gene expression activity and cell phenotype. Our current understanding is mainly derived from ensemble-level sequencing studies that reflect the 3D genome structure of millions of cells. These approaches have provided invaluable details on the 3D organizations of the genome and their relation to other nuclear landmarks. However, they mostly lack the ability to provide multimodal information simultaneously at the single-cell level. In recent years, cutting-edge imaging technologies have risen to the challenge of simultaneously describing multiple components of the nuclear space at the single-cell level, paving the way for a deeper understanding of the genome structure–function relationship. This review will focus on the development and utilization of such technologies to gain a multi-component view of the nucleus at single-cell resolution, dissecting the complexity and heterogeneity of nuclear organization.
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U2 - 10.1016/j.ceb.2023.102175
DO - 10.1016/j.ceb.2023.102175
M3 - Review article
C2 - 37263058
AN - SCOPUS:85162902329
SN - 0955-0674
VL - 82
JO - Current Opinion in Cell Biology
JF - Current Opinion in Cell Biology
M1 - 102175
ER -