Genomes of viral isolates derived from different mosquitos species

Mohammadreza Sadeghi, Vsevolod Popov, Hilda Guzman, Tung Gia Phan, Nikos Vasilakis, Robert Tesh, Eric Delwart

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Eleven viral isolates derived mostly in albopictus C6/36 cells from mosquito pools collected in Southeast Asia and the Americas between 1966 and 2014 contained particles with electron microscopy morphology typical of reoviruses. Metagenomics analysis yielded the near complete genomes of three novel reoviruses, Big Cypress orbivirus, Ninarumi virus, and High Island virus and a new tetravirus, Sarawak virus. Strains of previously characterized Sathuvarachi, Yunnan, Banna and Parry's Lagoon viruses (Reoviridae), Bontang virus (Mesoniviridae), and Culex theileri flavivirus (Flaviviridae) were also characterized. The availability of these mosquito virus genomes will facilitate their detection by metagenomics or PCR to better determine their geographic range, extent of host tropism, and possible association with arthropod or vertebrate disease.

Original languageEnglish (US)
Pages (from-to)49-57
Number of pages9
JournalVirus Research
Volume242
DOIs
StatePublished - Oct 15 2017

Fingerprint

Viral Genome
Culicidae
Viruses
Metagenomics
Orbivirus
Viral Tropism
Reoviridae
Flaviviridae
Cupressus
Genome
Flavivirus
Southeastern Asia
Culex
Arthropods
Malaysia
Islands
Vertebrates
Electron Microscopy
Polymerase Chain Reaction

Keywords

  • Deep sequencing
  • Metagenomics
  • Mosquito
  • Orbivirus
  • Reoviridae
  • Viral discovery

ASJC Scopus subject areas

  • Cancer Research
  • Infectious Diseases
  • Virology

Cite this

Genomes of viral isolates derived from different mosquitos species. / Sadeghi, Mohammadreza; Popov, Vsevolod; Guzman, Hilda; Phan, Tung Gia; Vasilakis, Nikos; Tesh, Robert; Delwart, Eric.

In: Virus Research, Vol. 242, 15.10.2017, p. 49-57.

Research output: Contribution to journalArticle

Sadeghi, Mohammadreza ; Popov, Vsevolod ; Guzman, Hilda ; Phan, Tung Gia ; Vasilakis, Nikos ; Tesh, Robert ; Delwart, Eric. / Genomes of viral isolates derived from different mosquitos species. In: Virus Research. 2017 ; Vol. 242. pp. 49-57.
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