Genomewide discovery and classification of candidate ovarian fertility genes in the mouse

Teresa D. Gallardo, George B. John, Lane Shirley, Cristina M. Contreras, Esra A. Akbay, J. Marshall Haynie, Samuel E. Ward, Meredith J. Shidler, Diego H. Castrillon

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Female infertility syndromes are among the most prevalent chronic health disorders in women, but their genetic basis remains unknown because of uncertainty regarding the number and identity of ovarian factors controlling the assembly, preservation, and maturation of ovarian follicles. To systematically discover ovarian fertility genes en masse, we employed a mouse model (Foxo3) in which follicles are assembled normally but then undergo synchronous activation. We developed a microarray-based approach for the systematic discovery of tissue-specific genes and, by applying it to Foxo3 ovaries and other samples, defined a surprisingly large set of ovarian factors (n = 348, ∼1% of the mouse genome). This set included the vast majority of known ovarian factors, 44% of which when mutated produce female sterility phenotypes, but most were novel. Comparative profiling of other tissues, including microdissected oocytes and somatic cells, revealed distinct gene classes and provided new insights into oogenesis and ovarian function, demonstrating the utility of our approach for tissue-specific gene discovery. This study will thus facilitate comprehensive analyses of follicle development, ovarian function, and female infertility.

Original languageEnglish (US)
Pages (from-to)179-194
Number of pages16
JournalGenetics
Volume177
Issue number1
DOIs
StatePublished - Sep 2007
Externally publishedYes

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Female Infertility
Fertility
Ovarian Follicle
Genes
Oogenesis
Genetic Association Studies
Uncertainty
Oocytes
Ovary
Genome
Phenotype
Health

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Gallardo, T. D., John, G. B., Shirley, L., Contreras, C. M., Akbay, E. A., Haynie, J. M., ... Castrillon, D. H. (2007). Genomewide discovery and classification of candidate ovarian fertility genes in the mouse. Genetics, 177(1), 179-194. https://doi.org/10.1534/genetics.107.074823

Genomewide discovery and classification of candidate ovarian fertility genes in the mouse. / Gallardo, Teresa D.; John, George B.; Shirley, Lane; Contreras, Cristina M.; Akbay, Esra A.; Haynie, J. Marshall; Ward, Samuel E.; Shidler, Meredith J.; Castrillon, Diego H.

In: Genetics, Vol. 177, No. 1, 09.2007, p. 179-194.

Research output: Contribution to journalArticle

Gallardo, TD, John, GB, Shirley, L, Contreras, CM, Akbay, EA, Haynie, JM, Ward, SE, Shidler, MJ & Castrillon, DH 2007, 'Genomewide discovery and classification of candidate ovarian fertility genes in the mouse', Genetics, vol. 177, no. 1, pp. 179-194. https://doi.org/10.1534/genetics.107.074823
Gallardo TD, John GB, Shirley L, Contreras CM, Akbay EA, Haynie JM et al. Genomewide discovery and classification of candidate ovarian fertility genes in the mouse. Genetics. 2007 Sep;177(1):179-194. https://doi.org/10.1534/genetics.107.074823
Gallardo, Teresa D. ; John, George B. ; Shirley, Lane ; Contreras, Cristina M. ; Akbay, Esra A. ; Haynie, J. Marshall ; Ward, Samuel E. ; Shidler, Meredith J. ; Castrillon, Diego H. / Genomewide discovery and classification of candidate ovarian fertility genes in the mouse. In: Genetics. 2007 ; Vol. 177, No. 1. pp. 179-194.
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