Genomic analysis of insulin-like growth factor-I gene transfer in thermally injured rats

Mohan R K Dasu, David Herndon, Marcus Spies, J. Regino Perez-Polo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Thermal trauma causes tissue damage by membrane destabilization and energy depletion at the cellular level, resulting in tissue necrosis and inflammation leading to delayed cell death. One therapeutic approach is to block the immediate triggering of the inflammatory cascade that results in prolonged hypermetabolic responses and immune dysfunction while promoting the expression of growth factors. In the present study, we determined hepatic gene expression responses to insulin-like growth factors-i (IGF-I) gene transfer to burned rats using high-density DNA microarray assays. The expression of 123 out of ∼8,800 genes assyed (1.4% of total) were significantly altered. Of these, 42 genes were altered irrespective of treatment by burn trauma (p < 0.05). Changes in gene expression were confirmed by measuring mRNA levels using reverse transcription-polymerase chain reaction and protein levels by Western blot assays. DNA microarray analyses showed two major mechanisms that mediated beneficial outcomes after IGF-I gene transfer in the burned rat livers. These mechanisms were the stimulation of IGF binding protein potentiation of peripheral IGF-I and the inhibition of the burn-augmented pro-apoptotic and oxidative mitochondrial metabolites stimulated by thermal trauma.

Original languageEnglish (US)
Pages (from-to)217-224
Number of pages8
JournalWound Repair and Regeneration
Volume12
Issue number2
DOIs
StatePublished - Mar 2004

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Insulin-Like Growth Factor I
Somatomedins
Oligonucleotide Array Sequence Analysis
Genes
Wounds and Injuries
Hot Temperature
Gene Expression
Insulin-Like Growth Factor Binding Proteins
Liver
Microarray Analysis
Burns
Reverse Transcription
Intercellular Signaling Peptides and Proteins
Cell Death
Necrosis
Western Blotting
Inflammation
Polymerase Chain Reaction
Messenger RNA
Membranes

ASJC Scopus subject areas

  • Dermatology
  • Surgery

Cite this

Genomic analysis of insulin-like growth factor-I gene transfer in thermally injured rats. / Dasu, Mohan R K; Herndon, David; Spies, Marcus; Perez-Polo, J. Regino.

In: Wound Repair and Regeneration, Vol. 12, No. 2, 03.2004, p. 217-224.

Research output: Contribution to journalArticle

Dasu, Mohan R K ; Herndon, David ; Spies, Marcus ; Perez-Polo, J. Regino. / Genomic analysis of insulin-like growth factor-I gene transfer in thermally injured rats. In: Wound Repair and Regeneration. 2004 ; Vol. 12, No. 2. pp. 217-224.
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