Genomic and phylogenetic characterization of viruses included in the Manzanilla and Oropouche species complexes of the genus Orthobunyavirus, family Bunyaviridae

Jason T. Ladner, Nazir Savji, Loreen Lofts, Amelia Travassos da Rosa, Michael R. Wiley, Marie C. Gestole, Gail E. Rosen, Hilda Guzman, Pedro F C Vasconcelos, Marcio R T Nunes, Tadeusz J. Kochel, W. Ian Lipkin, Robert B. Tesh, Gustavo Palacios

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Abstract

A thorough characterization of the genetic diversity of viruses present in vector and vertebrate host populations is essential for the early detection of and response to emerging pathogenic viruses, yet genetic characterization of many important viral groups remains incomplete. The Simbu serogroup of the genus Orthobunyavirus, family Bunyaviridae, is an example. The Simbu serogroup currently consists of a highly diverse group of related arboviruses that infect both humans and economically important livestock species. Here, we report complete genome sequences for 11 viruses within this group, with a focus on the large and poorly characterized Manzanilla and Oropouche species complexes. Phylogenetic and pairwise divergence analyses indicated the presence of high levels of genetic diversity within these two species complexes, on a par with that seen among the five other species complexes in the Simbu serogroup. Based on previously reported divergence thresholds between species, the data suggested that these two complexes should actually be divided into at least five species. Together these five species formed a distinct phylogenetic clade apart from the rest of the Simbu serogroup. Pairwise sequence divergences among viruses of this clade and viruses in other Simbu serogroup species complexes were similar to levels of divergence among the other orthobunyavirus serogroups. The genetic data also suggested relatively high levels of natural reassortment, with three potential reassortment events present, including two well-supported events involving viruses known to infect humans.

Original languageEnglish (US)
Pages (from-to)1055-1066
Number of pages12
JournalJournal of General Virology
Volume95
Issue numberPART 5
DOIs
StatePublished - 2014

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Orthobunyavirus
Bunyaviridae
Viruses
Arboviruses
Livestock
Vertebrates
Serogroup
Genome
Population

ASJC Scopus subject areas

  • Virology

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Genomic and phylogenetic characterization of viruses included in the Manzanilla and Oropouche species complexes of the genus Orthobunyavirus, family Bunyaviridae. / Ladner, Jason T.; Savji, Nazir; Lofts, Loreen; da Rosa, Amelia Travassos; Wiley, Michael R.; Gestole, Marie C.; Rosen, Gail E.; Guzman, Hilda; Vasconcelos, Pedro F C; Nunes, Marcio R T; Kochel, Tadeusz J.; Ian Lipkin, W.; Tesh, Robert B.; Palacios, Gustavo.

In: Journal of General Virology, Vol. 95, No. PART 5, 2014, p. 1055-1066.

Research output: Contribution to journalArticle

Ladner, JT, Savji, N, Lofts, L, da Rosa, AT, Wiley, MR, Gestole, MC, Rosen, GE, Guzman, H, Vasconcelos, PFC, Nunes, MRT, Kochel, TJ, Ian Lipkin, W, Tesh, RB & Palacios, G 2014, 'Genomic and phylogenetic characterization of viruses included in the Manzanilla and Oropouche species complexes of the genus Orthobunyavirus, family Bunyaviridae', Journal of General Virology, vol. 95, no. PART 5, pp. 1055-1066. https://doi.org/10.1099/vir.0.061309-0
Ladner, Jason T. ; Savji, Nazir ; Lofts, Loreen ; da Rosa, Amelia Travassos ; Wiley, Michael R. ; Gestole, Marie C. ; Rosen, Gail E. ; Guzman, Hilda ; Vasconcelos, Pedro F C ; Nunes, Marcio R T ; Kochel, Tadeusz J. ; Ian Lipkin, W. ; Tesh, Robert B. ; Palacios, Gustavo. / Genomic and phylogenetic characterization of viruses included in the Manzanilla and Oropouche species complexes of the genus Orthobunyavirus, family Bunyaviridae. In: Journal of General Virology. 2014 ; Vol. 95, No. PART 5. pp. 1055-1066.
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