Genotype 1a HCV (H77S) infection system.

Min Kyung Yi, Stanley M. Lemon

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

HCV is a small RNA virus belonging to the genus Hepacivirus within the virus family Flaviviridae. Infection with HCV often leads to chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. Current therapy, based on the use of interferon-alpha (IFN-alpha) in combination with ribavirin, results in limited success, especially in patients infected with the most prevalent genotype 1 viruses. Better therapies are needed, but the inability to propagate HCV in cell culture hampers antiviral drug-discovery efforts. Recently, fully permissive cell-culture systems have been developed that use viral RNA derived from the genotype 2a JFH-1 strain of HCV. Although these systems mark a significant breakthrough for HCV research, the parallel development of a tractable genotype 1a infection system (H77S virus) has provided significant advantages in assessing genotype 1-specific interventions, given the highly heterogeneous nature of HCV. H77S RNA contains five cell culture-adaptive mutations that are placed throughout the nonstructural protein-coding segment of the genome and render the RNA capable of robust replication in human hepatoma (Huh-7) cells. Although significantly less efficient than JFH-1 RNA, H77S RNA produces moderate titers of cell culture-infectious virus when transfected into Huh-7 cells.

Original languageEnglish (US)
Pages (from-to)337-346
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume510
StatePublished - 2009

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Cell Culture Techniques
Genotype
RNA
Viruses
Infection
Hepatocellular Carcinoma
Flaviviridae
Ribavirin
RNA Viruses
Viral RNA
Drug Discovery
Chronic Hepatitis
Interferon-alpha
Hepacivirus
Liver Cirrhosis
Antiviral Agents
Liver Diseases
Chronic Disease
Genome
Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Genotype 1a HCV (H77S) infection system. / Yi, Min Kyung; Lemon, Stanley M.

In: Methods in molecular biology (Clifton, N.J.), Vol. 510, 2009, p. 337-346.

Research output: Contribution to journalArticle

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