Genotypic and antigenic identification of two new strains of spotted fever group rickettsiae isolated from China

X. Yu, Y. Jin, M. Fan, G. Xu, Q. Liu, D. Raoult

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Four isolates of spotted fever group rickettsiae isolated from ticks in China were compared with all known species and strains of spotted fever group rickettsiae by immunofluorescence assay, DNA polymerase chain reaction followed by restriction endonuclease fragment length polymorphism analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and Western immunoblot. The Chinese isolates belonged to three types, including a novel serotype which has not been described before. One isolate obtained from tick ova of Dermacentor nuttallii in Inner Mongolia was antigenically and genotypically identical to Rickettsia sibirica. Two isolates obtained from Dermacentor sinicus collected from Beijing were identical, different from other members of spotted fever group rickettsiae but apparently closely related to R. sibirica. HA-91, a strain isolated from Hyalomma asiaticum bv. kozlovi olenew, was antigenically and genotypically unique among spotted fever group rickettsiae, and we feel that data presented here should prompt consideration of it as a new species on the basis of current rickettsial taxonomy.

Original languageEnglish (US)
Pages (from-to)83-88
Number of pages6
JournalJournal of Clinical Microbiology
Volume31
Issue number1
StatePublished - 1993
Externally publishedYes

Fingerprint

Rickettsia
China
Fever
Dermacentor
Ticks
DNA Restriction Enzymes
DNA-Directed DNA Polymerase
Sodium Dodecyl Sulfate
Restriction Fragment Length Polymorphisms
Fluorescent Antibody Technique
Ovum
Polyacrylamide Gel Electrophoresis
Western Blotting
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Genotypic and antigenic identification of two new strains of spotted fever group rickettsiae isolated from China. / Yu, X.; Jin, Y.; Fan, M.; Xu, G.; Liu, Q.; Raoult, D.

In: Journal of Clinical Microbiology, Vol. 31, No. 1, 1993, p. 83-88.

Research output: Contribution to journalArticle

Yu, X. ; Jin, Y. ; Fan, M. ; Xu, G. ; Liu, Q. ; Raoult, D. / Genotypic and antigenic identification of two new strains of spotted fever group rickettsiae isolated from China. In: Journal of Clinical Microbiology. 1993 ; Vol. 31, No. 1. pp. 83-88.
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