Genotypic Characterization of Environmental Isolates of Cryptococcus gattii from Puerto Rico

Yaliz Loperena-Alvarez, Ping Ren, Xiaojiang Li, D. J. Bopp, Alejandro Ruiz, Vishnu Chaturvedi, Carlos Rios-Velazquez

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Cryptococcus neoformans and Cryptococcus gattii are found in distinct environments with some overlap around different parts of the world. However, no systematic surveys of these two pathogens have been reported from Puerto Rico, a tropical island uniquely situated between mainland USA and countries in South America. We carried out an exhaustive environmental survey in southwestern Puerto Rico for pathogenic Cryptococcus species. Twenty-two presumptive isolates of C. gattii from cacti and tree detritus were characterized in detail by physiological and molecular methods and seventeen strains were confirmed as C. gattii. Cryptococcus gattii isolates were haploid and MAT strains. Sixteen out of seventeen C. gattii isolates belonged to VGII/AFLP6 genotype while one isolate was a VGIV/AFLP7 genotype. The results are significant as Puerto Rico strains are distinct from VGIII/AFLP5 strains reported from Southern California, but similar to C. gattii VGII/AFLP6 molecular type implicated in recent outbreaks of cryptococcosis in Pacific Northwest and British Columbia, Canada, but different in its M13 fingerprinting, and a common genotype in South America.

Original languageEnglish (US)
Pages (from-to)279-285
Number of pages7
JournalMycopathologia
Volume170
Issue number4
DOIs
StatePublished - 2010
Externally publishedYes

Fingerprint

Cryptococcus gattii
Puerto Rico
South America
Genotype
genotype
Northwestern United States
Cactaceae
Cryptococcus (Tremellomycetes)
Cryptococcus
Cryptococcosis
British Columbia
Cryptococcus neoformans
Haploidy
Islands
haploidy
Canada
Disease Outbreaks
pathogens

Keywords

  • C. gattii
  • Environmental isolates
  • Puerto Rico

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Applied Microbiology and Biotechnology
  • Microbiology
  • veterinary (miscalleneous)

Cite this

Loperena-Alvarez, Y., Ren, P., Li, X., Bopp, D. J., Ruiz, A., Chaturvedi, V., & Rios-Velazquez, C. (2010). Genotypic Characterization of Environmental Isolates of Cryptococcus gattii from Puerto Rico. Mycopathologia, 170(4), 279-285. https://doi.org/10.1007/s11046-010-9296-3

Genotypic Characterization of Environmental Isolates of Cryptococcus gattii from Puerto Rico. / Loperena-Alvarez, Yaliz; Ren, Ping; Li, Xiaojiang; Bopp, D. J.; Ruiz, Alejandro; Chaturvedi, Vishnu; Rios-Velazquez, Carlos.

In: Mycopathologia, Vol. 170, No. 4, 2010, p. 279-285.

Research output: Contribution to journalArticle

Loperena-Alvarez, Y, Ren, P, Li, X, Bopp, DJ, Ruiz, A, Chaturvedi, V & Rios-Velazquez, C 2010, 'Genotypic Characterization of Environmental Isolates of Cryptococcus gattii from Puerto Rico', Mycopathologia, vol. 170, no. 4, pp. 279-285. https://doi.org/10.1007/s11046-010-9296-3
Loperena-Alvarez, Yaliz ; Ren, Ping ; Li, Xiaojiang ; Bopp, D. J. ; Ruiz, Alejandro ; Chaturvedi, Vishnu ; Rios-Velazquez, Carlos. / Genotypic Characterization of Environmental Isolates of Cryptococcus gattii from Puerto Rico. In: Mycopathologia. 2010 ; Vol. 170, No. 4. pp. 279-285.
@article{3d056147722241d7adf114b65edab0ff,
title = "Genotypic Characterization of Environmental Isolates of Cryptococcus gattii from Puerto Rico",
abstract = "Cryptococcus neoformans and Cryptococcus gattii are found in distinct environments with some overlap around different parts of the world. However, no systematic surveys of these two pathogens have been reported from Puerto Rico, a tropical island uniquely situated between mainland USA and countries in South America. We carried out an exhaustive environmental survey in southwestern Puerto Rico for pathogenic Cryptococcus species. Twenty-two presumptive isolates of C. gattii from cacti and tree detritus were characterized in detail by physiological and molecular methods and seventeen strains were confirmed as C. gattii. Cryptococcus gattii isolates were haploid and MAT strains. Sixteen out of seventeen C. gattii isolates belonged to VGII/AFLP6 genotype while one isolate was a VGIV/AFLP7 genotype. The results are significant as Puerto Rico strains are distinct from VGIII/AFLP5 strains reported from Southern California, but similar to C. gattii VGII/AFLP6 molecular type implicated in recent outbreaks of cryptococcosis in Pacific Northwest and British Columbia, Canada, but different in its M13 fingerprinting, and a common genotype in South America.",
keywords = "C. gattii, Environmental isolates, Puerto Rico",
author = "Yaliz Loperena-Alvarez and Ping Ren and Xiaojiang Li and Bopp, {D. J.} and Alejandro Ruiz and Vishnu Chaturvedi and Carlos Rios-Velazquez",
year = "2010",
doi = "10.1007/s11046-010-9296-3",
language = "English (US)",
volume = "170",
pages = "279--285",
journal = "Mycopathologia",
issn = "0301-486X",
publisher = "Springer Netherlands",
number = "4",

}

TY - JOUR

T1 - Genotypic Characterization of Environmental Isolates of Cryptococcus gattii from Puerto Rico

AU - Loperena-Alvarez, Yaliz

AU - Ren, Ping

AU - Li, Xiaojiang

AU - Bopp, D. J.

AU - Ruiz, Alejandro

AU - Chaturvedi, Vishnu

AU - Rios-Velazquez, Carlos

PY - 2010

Y1 - 2010

N2 - Cryptococcus neoformans and Cryptococcus gattii are found in distinct environments with some overlap around different parts of the world. However, no systematic surveys of these two pathogens have been reported from Puerto Rico, a tropical island uniquely situated between mainland USA and countries in South America. We carried out an exhaustive environmental survey in southwestern Puerto Rico for pathogenic Cryptococcus species. Twenty-two presumptive isolates of C. gattii from cacti and tree detritus were characterized in detail by physiological and molecular methods and seventeen strains were confirmed as C. gattii. Cryptococcus gattii isolates were haploid and MAT strains. Sixteen out of seventeen C. gattii isolates belonged to VGII/AFLP6 genotype while one isolate was a VGIV/AFLP7 genotype. The results are significant as Puerto Rico strains are distinct from VGIII/AFLP5 strains reported from Southern California, but similar to C. gattii VGII/AFLP6 molecular type implicated in recent outbreaks of cryptococcosis in Pacific Northwest and British Columbia, Canada, but different in its M13 fingerprinting, and a common genotype in South America.

AB - Cryptococcus neoformans and Cryptococcus gattii are found in distinct environments with some overlap around different parts of the world. However, no systematic surveys of these two pathogens have been reported from Puerto Rico, a tropical island uniquely situated between mainland USA and countries in South America. We carried out an exhaustive environmental survey in southwestern Puerto Rico for pathogenic Cryptococcus species. Twenty-two presumptive isolates of C. gattii from cacti and tree detritus were characterized in detail by physiological and molecular methods and seventeen strains were confirmed as C. gattii. Cryptococcus gattii isolates were haploid and MAT strains. Sixteen out of seventeen C. gattii isolates belonged to VGII/AFLP6 genotype while one isolate was a VGIV/AFLP7 genotype. The results are significant as Puerto Rico strains are distinct from VGIII/AFLP5 strains reported from Southern California, but similar to C. gattii VGII/AFLP6 molecular type implicated in recent outbreaks of cryptococcosis in Pacific Northwest and British Columbia, Canada, but different in its M13 fingerprinting, and a common genotype in South America.

KW - C. gattii

KW - Environmental isolates

KW - Puerto Rico

UR - http://www.scopus.com/inward/record.url?scp=77956409507&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956409507&partnerID=8YFLogxK

U2 - 10.1007/s11046-010-9296-3

DO - 10.1007/s11046-010-9296-3

M3 - Article

C2 - 20306144

AN - SCOPUS:77956409507

VL - 170

SP - 279

EP - 285

JO - Mycopathologia

JF - Mycopathologia

SN - 0301-486X

IS - 4

ER -