Genotipificación y evaluación de la dinámica de infección de un aislamiento colombiano de Leptospira santarosai en el modelo experimental en hámster

Translated title of the contribution: Genotyping and evaluation of infection dynamics in a Colombian isolate of Leptospira santarosai in hamster as an experimental model

Piedad Agudelo-Flórez, Harold Durango, Diego Aranzazu, Juan D avid Rodas, Bruno Travi

Research output: Contribution to journalArticle

Abstract

INTRODUCTION: Is necessary to develop models for the study of leptospirosis.

OBJECTIVE: To genotype a Colombian strain of Leptospira isolated from a human with Weil´s syndrome and to evaluate its infection dynamics in the hamster experimental model.

MATERIALS AND METHODS: Genotyping was performed by amplification and sequence analysis of the rrs 16S and lipL32 genes. The median lethal dose was determined in intraperitoneally inoculated hamsters. The patterns of clinical chemistry, the duration of leptospiremia, leptospiruria and pathological findings were studied and compared in the same animal model infected with L. interrogans (Fiocruz L1-130).

RESULTS: Molecular typing revealed that the isolate corresponded to the pathogenic species L. santarosai, which was recovered from hamsters´ kidneys and lungs and detected by lipL32 PCR from day 3 post-infection in these organs. There was a marked increase of C-reactive protein in animals at day 5 post-infection (3.25 mg/dl; normal value: 0.3 mg/dl) with decreases by day 18 (2.60 mg/dl: normal value: 0.8 mg/dl). Biomarkers of urea showed changes consistent with possible renal acute failure (day 5 post-infection: 49.01 mg/dl and day 18 post-infection: 53.71 mg/dl). Histopathological changes included interstitial pneumonia with varying degrees of hemorrhage and interstitial nephritis.

CONCLUSION: The pathogenic species L. santarosai was identified in Colombia. Its pathogenicity as determined by tropism to lung and kidney was comparable to that of L. interrogans Fiocruz L1-130, well known for its virulence and pulmonar tropism. The biological aspects studied here had never before been evaluated in an autochthonous isolate.

Original languageSpanish
Pages (from-to)460-472
Number of pages13
JournalBiomédica : revista del Instituto Nacional de Salud
Volume34
Issue number3
DOIs
StatePublished - Jul 1 2014

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Leptospira
Cricetinae
Theoretical Models
Animals
Infection
Tropism
Biomarkers
Virulence
Reference Values
C-Reactive Protein
Amplification
Urea
Kidney
Molecular Typing
Genes
Interstitial Nephritis
Lung
Leptospirosis
Clinical Chemistry
Colombia

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Genotipificación y evaluación de la dinámica de infección de un aislamiento colombiano de Leptospira santarosai en el modelo experimental en hámster. / Agudelo-Flórez, Piedad; Durango, Harold; Aranzazu, Diego; Rodas, Juan D avid; Travi, Bruno.

In: Biomédica : revista del Instituto Nacional de Salud, Vol. 34, No. 3, 01.07.2014, p. 460-472.

Research output: Contribution to journalArticle

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