Geographic classification of dengue-2 virus strains by antigen signature analysis

T. P. Monath, J. R. Wands, L. J. Hill, N. V. Brown, R. A. Marciniak, M. A. Wong, M. K. Gentry, D. S. Burke, J. A. Grant, D. W. Trent

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Dengue-2 virus strains from different locations were compared by T1-RNAse-resistant oligonucleotide fingerprinting and antigen signature analysis. The latter technique involved construction of radioimmunoassays using monoclonal antibodies that recognize nine distinct dengue-2 type-specific and flavivirus cross-reactive epitopes over a range of antigen concentrations. A statistical method was used to align unknown dengue antigen concentrations in different strain preparations, allowing comparison of binding profiles. Twentysix dengue-2 virus strains were separated into five distinct groups (topotypes) on the basis of unique RNA fingerprints. Two of these were represented by New Guinea C, the prototype virus isolated in 1944, and a Philippine strain; others were segregated on the basis of ≥80% shared oligonucleotides into similarity groups representing Burma/Thailand (8 strains), Puerto Rico (12 strains), and Jamaica (4 strains). Signature analysis of the prototype and four geographic topotype strains revealed striking antigenic differences. In contrast, a high degree of antigenic similarity was found among strains from the same geographic region. Variation between antigenically distinct strains occurred at both type-specific and group-reactive epitopes, but the widest differences appeared at group-reactive determinants. Signature analysis provides a more rapid and simpler means than RNA fingerprinting of monitoring changes or new introductions of dengue virus populations in a geographic region.

Original languageEnglish (US)
Pages (from-to)313-324
Number of pages12
JournalVirology
Volume154
Issue number2
DOIs
StatePublished - Oct 30 1986

Fingerprint

Dengue Virus
Dengue
Antigens
Oligonucleotides
Epitopes
New Guinea
RNA
Myanmar
Jamaica
Flavivirus
Puerto Rico
Philippines
Dermatoglyphics
Thailand
Radioimmunoassay
Monoclonal Antibodies
Viruses
Population

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Monath, T. P., Wands, J. R., Hill, L. J., Brown, N. V., Marciniak, R. A., Wong, M. A., ... Trent, D. W. (1986). Geographic classification of dengue-2 virus strains by antigen signature analysis. Virology, 154(2), 313-324. https://doi.org/10.1016/0042-6822(86)90457-5

Geographic classification of dengue-2 virus strains by antigen signature analysis. / Monath, T. P.; Wands, J. R.; Hill, L. J.; Brown, N. V.; Marciniak, R. A.; Wong, M. A.; Gentry, M. K.; Burke, D. S.; Grant, J. A.; Trent, D. W.

In: Virology, Vol. 154, No. 2, 30.10.1986, p. 313-324.

Research output: Contribution to journalArticle

Monath, TP, Wands, JR, Hill, LJ, Brown, NV, Marciniak, RA, Wong, MA, Gentry, MK, Burke, DS, Grant, JA & Trent, DW 1986, 'Geographic classification of dengue-2 virus strains by antigen signature analysis', Virology, vol. 154, no. 2, pp. 313-324. https://doi.org/10.1016/0042-6822(86)90457-5
Monath TP, Wands JR, Hill LJ, Brown NV, Marciniak RA, Wong MA et al. Geographic classification of dengue-2 virus strains by antigen signature analysis. Virology. 1986 Oct 30;154(2):313-324. https://doi.org/10.1016/0042-6822(86)90457-5
Monath, T. P. ; Wands, J. R. ; Hill, L. J. ; Brown, N. V. ; Marciniak, R. A. ; Wong, M. A. ; Gentry, M. K. ; Burke, D. S. ; Grant, J. A. ; Trent, D. W. / Geographic classification of dengue-2 virus strains by antigen signature analysis. In: Virology. 1986 ; Vol. 154, No. 2. pp. 313-324.
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