TY - JOUR
T1 - Germinal centre and marginal zone B cells expand quickly in a second Plasmodium chabaudi malaria infection producing mature plasma cells
AU - Stephens, R.
AU - Ndungu, F. M.
AU - Langhorne, J.
PY - 2009/1
Y1 - 2009/1
N2 - Antibodies and B cells are critical in the protective immune response to the blood stage of the malaria parasite, Plasmodium chabaudi. However, little is known about the development of memory B cells and their differentiation into plasma cells during infection or after re-infection. Here we have shown that B cells with phenotypic characteristics of memory cells (CD19+IgD - CD38+, IgG1+) are generated in a primary Plasmodium chabaudi chabaudi infection of mice. In addition, we observed that germinal centre cells (CD19+, GL7+, MHCIIhi) and Marginal Zone B cells (CD19+CD23-IgD-) show faster expansion on re-infection than in the primary, though other subsets do not. Interestingly, though both IgM- and IgM+ memory cells are produced, IgM+ memory cells do not expand on second infection. The second infection quickly produced mature bone marrow plasma cells (intracellular Ighi, CD138hi, CD9+, B220 -), compared to primary infection; which generates a very large population of immature splenic plasma cells (B220+). This analysis suggests that a memory B cell population is generated after a single infection of malaria, which on re-infection responds quickly producing germinal centres and generating long-lived plasma cells making the second encounter with parasite more efficient.
AB - Antibodies and B cells are critical in the protective immune response to the blood stage of the malaria parasite, Plasmodium chabaudi. However, little is known about the development of memory B cells and their differentiation into plasma cells during infection or after re-infection. Here we have shown that B cells with phenotypic characteristics of memory cells (CD19+IgD - CD38+, IgG1+) are generated in a primary Plasmodium chabaudi chabaudi infection of mice. In addition, we observed that germinal centre cells (CD19+, GL7+, MHCIIhi) and Marginal Zone B cells (CD19+CD23-IgD-) show faster expansion on re-infection than in the primary, though other subsets do not. Interestingly, though both IgM- and IgM+ memory cells are produced, IgM+ memory cells do not expand on second infection. The second infection quickly produced mature bone marrow plasma cells (intracellular Ighi, CD138hi, CD9+, B220 -), compared to primary infection; which generates a very large population of immature splenic plasma cells (B220+). This analysis suggests that a memory B cell population is generated after a single infection of malaria, which on re-infection responds quickly producing germinal centres and generating long-lived plasma cells making the second encounter with parasite more efficient.
KW - B lymphocyte
KW - Immunological memory
KW - Malaria
KW - Mouse
UR - http://www.scopus.com/inward/record.url?scp=57649233027&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=57649233027&partnerID=8YFLogxK
U2 - 10.1111/j.1365-3024.2008.01066.x
DO - 10.1111/j.1365-3024.2008.01066.x
M3 - Article
C2 - 19121080
AN - SCOPUS:57649233027
SN - 0141-9838
VL - 31
SP - 20
EP - 31
JO - Parasite Immunology
JF - Parasite Immunology
IS - 1
ER -