Gestational changes in calcitonin gene-related peptide, nerve growth factor, and its receptors in rat dorsal root ganglia

P. Lanlua, P. R R Gangula, Giulio Taglialatela, C. Yallampalli

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In dorsal root ganglia (DRG) cell cultures, levels of calcitonin gene-related peptide (CGRP) are increased in the presence of ovarian hormones and nerve growth factor (NGF). In addition, injection of ovariectomized rats with ovarian hormones led to an increase in levels of two NGF receptors, TrkA and p75NTR, in DRG. Thus, we hypothesized that increased levels of ovarian hormones during pregnancy may elevate the synthesis of CGRP and NGF receptors in the DRG. DRG harvested from rats on specific days of pregnancy, on Day 2 postpartum, and after ovariectomy were subjected to radioimmunoassay, Western blot analysis, and NGF immunoassay to determine levels of CGRP, TrkA and p75NTR, and NGF, respectively. CGRP levels in rat DRG were significantly higher during pregnancy than at Day 2 post-partum or in ovariectomized rats. Levels of both TrkA and p75NTR in DRG increased during pregnancy and remained elevated at Day 2 postpartum, but CGRP levels declined. Levels of NGF reached a statistically significant peak at Day 18 of gestation, and were not significantly reduced at Day 2 postpartum. Increased levels of ovarian steroid hormones during pregnancy may be involved in the synthesis of CGRP, however, the post-partum decreases in CGRP synthesis appear to be unrelated to NGF and its receptors.

Original languageEnglish (US)
Pages (from-to)1601-1605
Number of pages5
JournalBiology of Reproduction
Volume65
Issue number5
StatePublished - 2001

Fingerprint

Nerve Growth Factor Receptors
Calcitonin Gene-Related Peptide
Spinal Ganglia
Nerve Growth Factor
Pregnancy
Hormones
Postpartum Period
Ovariectomy
Immunoassay
Radioimmunoassay
Cell Culture Techniques
Western Blotting
Steroids
Injections

Keywords

  • Estradiol
  • Growth factors
  • Pregnancy
  • Progesterone

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Gestational changes in calcitonin gene-related peptide, nerve growth factor, and its receptors in rat dorsal root ganglia. / Lanlua, P.; Gangula, P. R R; Taglialatela, Giulio; Yallampalli, C.

In: Biology of Reproduction, Vol. 65, No. 5, 2001, p. 1601-1605.

Research output: Contribution to journalArticle

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