Global profiling of Streptococcus pneumoniae gene expression at different growth temperatures

Utpal Pandya, Christopher A. Allen, David A. Watson, David Niesel

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Streptococcus pneumoniae is a common commensal of the upper respiratory tract of healthy humans and is an important pathogen in young children, immunocompromised adults, and the elderly. To better understand the strategies employed by this bacterial species in adapting to conditions present at different infection sites in the host, global transcription profiling was used to study gene expression at different growth temperatures: 21, 29, 33, 37, and 40 °C. Here, we found that 658 genes (29%) out of 1717 genes were differently expressed (≥1.5-fold change) in at least one growth temperature relative to 37°C. The percentages of genes whose expression was altered in each growth temperature, respectively, were: 21°C: 53% ↑, 47% ↓ 29°C: 44% ↑, 56% ↓; 33°C: 27% ↑, 73% ↓ and 40°C: 44% ↑, 56% ↓. Hierarchical clustering (HC) of the temperature regulated genes resulted in four clusters, namely A-D of differently expressed genes grouped by bacterial growth temperature. Cluster A represented 81 genes reflecting enhanced expression at 33°C. Cluster B included 260 genes whose expression increased with growth temperature. Cluster C had 28 genes with 68% showing enhanced expression at 29°C while cluster D had 289 genes with 74% genes showing enhanced expression at 21°C relative to 37°C. Principal component (PC) analysis also divided differentially expressed genes into four groups and was highly correlated with HC, suggesting that temperature regulated expression is not random but coordinated. Overall, these results indicated substantial reprogramming of transcription in response to growth temperature. Functional characterization of differential gene expression at different temperatures provides further information on the molecular mechanism(s) that allows S. pneumoniae to adapt to various host environments.

Original languageEnglish (US)
Pages (from-to)45-54
Number of pages10
JournalGene
Volume360
Issue number1
DOIs
StatePublished - Oct 24 2005

Fingerprint

Streptococcus pneumoniae
Gene Expression
Temperature
Growth
Genes
Cluster Analysis
Bacterial Genes
Principal Component Analysis
Respiratory System
Infection

Keywords

  • DNA microarray
  • Gene expression
  • Temperature regulation

ASJC Scopus subject areas

  • Genetics

Cite this

Global profiling of Streptococcus pneumoniae gene expression at different growth temperatures. / Pandya, Utpal; Allen, Christopher A.; Watson, David A.; Niesel, David.

In: Gene, Vol. 360, No. 1, 24.10.2005, p. 45-54.

Research output: Contribution to journalArticle

Pandya, Utpal ; Allen, Christopher A. ; Watson, David A. ; Niesel, David. / Global profiling of Streptococcus pneumoniae gene expression at different growth temperatures. In: Gene. 2005 ; Vol. 360, No. 1. pp. 45-54.
@article{135ac50245db4871a6912ef76a674ae5,
title = "Global profiling of Streptococcus pneumoniae gene expression at different growth temperatures",
abstract = "Streptococcus pneumoniae is a common commensal of the upper respiratory tract of healthy humans and is an important pathogen in young children, immunocompromised adults, and the elderly. To better understand the strategies employed by this bacterial species in adapting to conditions present at different infection sites in the host, global transcription profiling was used to study gene expression at different growth temperatures: 21, 29, 33, 37, and 40 °C. Here, we found that 658 genes (29{\%}) out of 1717 genes were differently expressed (≥1.5-fold change) in at least one growth temperature relative to 37°C. The percentages of genes whose expression was altered in each growth temperature, respectively, were: 21°C: 53{\%} ↑, 47{\%} ↓ 29°C: 44{\%} ↑, 56{\%} ↓; 33°C: 27{\%} ↑, 73{\%} ↓ and 40°C: 44{\%} ↑, 56{\%} ↓. Hierarchical clustering (HC) of the temperature regulated genes resulted in four clusters, namely A-D of differently expressed genes grouped by bacterial growth temperature. Cluster A represented 81 genes reflecting enhanced expression at 33°C. Cluster B included 260 genes whose expression increased with growth temperature. Cluster C had 28 genes with 68{\%} showing enhanced expression at 29°C while cluster D had 289 genes with 74{\%} genes showing enhanced expression at 21°C relative to 37°C. Principal component (PC) analysis also divided differentially expressed genes into four groups and was highly correlated with HC, suggesting that temperature regulated expression is not random but coordinated. Overall, these results indicated substantial reprogramming of transcription in response to growth temperature. Functional characterization of differential gene expression at different temperatures provides further information on the molecular mechanism(s) that allows S. pneumoniae to adapt to various host environments.",
keywords = "DNA microarray, Gene expression, Temperature regulation",
author = "Utpal Pandya and Allen, {Christopher A.} and Watson, {David A.} and David Niesel",
year = "2005",
month = "10",
day = "24",
doi = "10.1016/j.gene.2005.06.023",
language = "English (US)",
volume = "360",
pages = "45--54",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Global profiling of Streptococcus pneumoniae gene expression at different growth temperatures

AU - Pandya, Utpal

AU - Allen, Christopher A.

AU - Watson, David A.

AU - Niesel, David

PY - 2005/10/24

Y1 - 2005/10/24

N2 - Streptococcus pneumoniae is a common commensal of the upper respiratory tract of healthy humans and is an important pathogen in young children, immunocompromised adults, and the elderly. To better understand the strategies employed by this bacterial species in adapting to conditions present at different infection sites in the host, global transcription profiling was used to study gene expression at different growth temperatures: 21, 29, 33, 37, and 40 °C. Here, we found that 658 genes (29%) out of 1717 genes were differently expressed (≥1.5-fold change) in at least one growth temperature relative to 37°C. The percentages of genes whose expression was altered in each growth temperature, respectively, were: 21°C: 53% ↑, 47% ↓ 29°C: 44% ↑, 56% ↓; 33°C: 27% ↑, 73% ↓ and 40°C: 44% ↑, 56% ↓. Hierarchical clustering (HC) of the temperature regulated genes resulted in four clusters, namely A-D of differently expressed genes grouped by bacterial growth temperature. Cluster A represented 81 genes reflecting enhanced expression at 33°C. Cluster B included 260 genes whose expression increased with growth temperature. Cluster C had 28 genes with 68% showing enhanced expression at 29°C while cluster D had 289 genes with 74% genes showing enhanced expression at 21°C relative to 37°C. Principal component (PC) analysis also divided differentially expressed genes into four groups and was highly correlated with HC, suggesting that temperature regulated expression is not random but coordinated. Overall, these results indicated substantial reprogramming of transcription in response to growth temperature. Functional characterization of differential gene expression at different temperatures provides further information on the molecular mechanism(s) that allows S. pneumoniae to adapt to various host environments.

AB - Streptococcus pneumoniae is a common commensal of the upper respiratory tract of healthy humans and is an important pathogen in young children, immunocompromised adults, and the elderly. To better understand the strategies employed by this bacterial species in adapting to conditions present at different infection sites in the host, global transcription profiling was used to study gene expression at different growth temperatures: 21, 29, 33, 37, and 40 °C. Here, we found that 658 genes (29%) out of 1717 genes were differently expressed (≥1.5-fold change) in at least one growth temperature relative to 37°C. The percentages of genes whose expression was altered in each growth temperature, respectively, were: 21°C: 53% ↑, 47% ↓ 29°C: 44% ↑, 56% ↓; 33°C: 27% ↑, 73% ↓ and 40°C: 44% ↑, 56% ↓. Hierarchical clustering (HC) of the temperature regulated genes resulted in four clusters, namely A-D of differently expressed genes grouped by bacterial growth temperature. Cluster A represented 81 genes reflecting enhanced expression at 33°C. Cluster B included 260 genes whose expression increased with growth temperature. Cluster C had 28 genes with 68% showing enhanced expression at 29°C while cluster D had 289 genes with 74% genes showing enhanced expression at 21°C relative to 37°C. Principal component (PC) analysis also divided differentially expressed genes into four groups and was highly correlated with HC, suggesting that temperature regulated expression is not random but coordinated. Overall, these results indicated substantial reprogramming of transcription in response to growth temperature. Functional characterization of differential gene expression at different temperatures provides further information on the molecular mechanism(s) that allows S. pneumoniae to adapt to various host environments.

KW - DNA microarray

KW - Gene expression

KW - Temperature regulation

UR - http://www.scopus.com/inward/record.url?scp=26444495536&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=26444495536&partnerID=8YFLogxK

U2 - 10.1016/j.gene.2005.06.023

DO - 10.1016/j.gene.2005.06.023

M3 - Article

C2 - 16154298

AN - SCOPUS:26444495536

VL - 360

SP - 45

EP - 54

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1

ER -