Global transcriptome analysis of Aedes aegypti mosquitoes in response to Zika virus infection

Kayvan Etebari, Shivanand Hegde, Miguel A. Saldaña, Steven Widen, Thomas Wood, Sassan Asgari, Grant L. Hughes

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Zika virus (ZIKV) of the Flaviviridae family is a recently emerged mosquitoborne virus that has been implicated in the surge of the number of microcephaly instances in South America. The recent rapid spread of the virus led to its declaration as a global health emergency by the World Health Organization. The virus is transmitted mainly by the mosquito Aedes aegypti, which is also the vector of dengue virus; however, little is known about the interactions of the virus with the mosquito vector. In this study, we investigated the transcriptome profiles of whole A. aegypti mosquitoes in response to ZIKV infection at 2, 7, and 14 days postinfection using transcriptome sequencing. Results showed changes in the abundance of a large number of transcripts at each time point following infection, with 18 transcripts commonly changed among the three time points. Gene ontology analysis revealed that most of the altered genes are involved in metabolic processes, cellular processes, and proteolysis. In addition, 486 long intergenic noncoding RNAs that were altered upon ZIKV infection were identified. Further, we found changes of a number of potential mRNA target genes correlating with those of altered host microRNAs. The outcomes provide a basic understanding of A. aegypti responses to ZIKV and help to determine host factors involved in replication or mosquito host antiviral response against the virus.

Original languageEnglish (US)
Article numbere00456-17
JournalmSphere
Volume2
Issue number6
DOIs
StatePublished - Nov 1 2017

Fingerprint

Aedes
Gene Expression Profiling
Culicidae
Viruses
Transcriptome
Flaviviridae
Long Noncoding RNA
Microcephaly
Dengue Virus
Gene Ontology
South America
MicroRNAs
Proteolysis
Genes
Antiviral Agents
Emergencies
Zika Virus Infection
Messenger RNA
Infection
Zika Virus

Keywords

  • Aedes aegypti
  • Behavior
  • Long noncoding RNA
  • MicroRNA
  • Odorant binding protein
  • RNA-Seq
  • Transcriptome
  • Zika virus

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Etebari, K., Hegde, S., Saldaña, M. A., Widen, S., Wood, T., Asgari, S., & Hughes, G. L. (2017). Global transcriptome analysis of Aedes aegypti mosquitoes in response to Zika virus infection. mSphere, 2(6), [e00456-17]. https://doi.org/10.1128/mSphere.00456-17

Global transcriptome analysis of Aedes aegypti mosquitoes in response to Zika virus infection. / Etebari, Kayvan; Hegde, Shivanand; Saldaña, Miguel A.; Widen, Steven; Wood, Thomas; Asgari, Sassan; Hughes, Grant L.

In: mSphere, Vol. 2, No. 6, e00456-17, 01.11.2017.

Research output: Contribution to journalArticle

Etebari, K, Hegde, S, Saldaña, MA, Widen, S, Wood, T, Asgari, S & Hughes, GL 2017, 'Global transcriptome analysis of Aedes aegypti mosquitoes in response to Zika virus infection', mSphere, vol. 2, no. 6, e00456-17. https://doi.org/10.1128/mSphere.00456-17
Etebari, Kayvan ; Hegde, Shivanand ; Saldaña, Miguel A. ; Widen, Steven ; Wood, Thomas ; Asgari, Sassan ; Hughes, Grant L. / Global transcriptome analysis of Aedes aegypti mosquitoes in response to Zika virus infection. In: mSphere. 2017 ; Vol. 2, No. 6.
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