Global transcriptomic profiling of Schizosaccharomyces pombe in response to nitrosative stress

Pranjal Biswas, Sanjay Ghosh

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Excess production of nitric oxide (NO) and reactive nitrogen intermediates (RNIs) cause nitrosative stress on cells. Schizosaccharomyces pombe was used as a model to study nitrosative stress response. This is the first report on the global gene expression profile in response to NO in S. pombe using microarray. Among the 4824 genes reported for S. pombe, 818 were differentially expressed by at least 2-fold upon NO donor treatment. We previously showed that Pap1, the Activator Protein 1 transcription factor is required to combat nitrosative stress. In this study, the transcriptional response to NO in a null mutant for pap1 identified 45 genes that seem to be controlled by Pap1. Surprisingly, Pap1 regulated genes in S. pombe were distinctly different under nitrosative stress than those reported under oxidative stress. Genes of the pathway meiosis, cell cycle, spliceosome and oxidative phosphorylation were mostly affected under nitrosative stress in the fission yeast.

Original languageEnglish (US)
Pages (from-to)241-253
Number of pages13
Issue number2
StatePublished - Mar 10 2015
Externally publishedYes


  • Microarray analysis
  • Nitric oxide
  • Nitrosative stress
  • Schizosaccharomyces pombe

ASJC Scopus subject areas

  • Genetics


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