TY - JOUR
T1 - Global transcriptomic profiling of Schizosaccharomyces pombe in response to nitrosative stress
AU - Biswas, Pranjal
AU - Ghosh, Sanjay
N1 - Funding Information:
The authors would like to acknowledge the generous support of Dr. Elena Hidalgo (Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Barcelona, Spain) for kindly providing the yeast strains, Dr. Sandipan Ganguly (Division of Parasitology, National Institute of Cholera and Enteric Diseases, Kolkata, India) for providing the real time PCR facility. We also thank Mr. Dibyendu Raj, research fellow in Dr. Ganguly's laboratory, for his help during the real time PCR experiments. We thank members of our lab for the discussion. We thank all the funding agencies for their financial support. The consumable for the entire work was supported by DBT, Govt. of India , Grant No. BT/PR12551/BRB/10/02/2009 dated September 3, 2010, DBT-IPLS, UPE and UGC CAS Phase II Govt. of India for providing infrastructural facility, and CSIR, Govt. of India for providing fellowship to PB. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© 2014 Elsevier B.V.
PY - 2015/3/10
Y1 - 2015/3/10
N2 - Excess production of nitric oxide (NO) and reactive nitrogen intermediates (RNIs) cause nitrosative stress on cells. Schizosaccharomyces pombe was used as a model to study nitrosative stress response. This is the first report on the global gene expression profile in response to NO in S. pombe using microarray. Among the 4824 genes reported for S. pombe, 818 were differentially expressed by at least 2-fold upon NO donor treatment. We previously showed that Pap1, the Activator Protein 1 transcription factor is required to combat nitrosative stress. In this study, the transcriptional response to NO in a null mutant for pap1 identified 45 genes that seem to be controlled by Pap1. Surprisingly, Pap1 regulated genes in S. pombe were distinctly different under nitrosative stress than those reported under oxidative stress. Genes of the pathway meiosis, cell cycle, spliceosome and oxidative phosphorylation were mostly affected under nitrosative stress in the fission yeast.
AB - Excess production of nitric oxide (NO) and reactive nitrogen intermediates (RNIs) cause nitrosative stress on cells. Schizosaccharomyces pombe was used as a model to study nitrosative stress response. This is the first report on the global gene expression profile in response to NO in S. pombe using microarray. Among the 4824 genes reported for S. pombe, 818 were differentially expressed by at least 2-fold upon NO donor treatment. We previously showed that Pap1, the Activator Protein 1 transcription factor is required to combat nitrosative stress. In this study, the transcriptional response to NO in a null mutant for pap1 identified 45 genes that seem to be controlled by Pap1. Surprisingly, Pap1 regulated genes in S. pombe were distinctly different under nitrosative stress than those reported under oxidative stress. Genes of the pathway meiosis, cell cycle, spliceosome and oxidative phosphorylation were mostly affected under nitrosative stress in the fission yeast.
KW - Microarray analysis
KW - Nitric oxide
KW - Nitrosative stress
KW - Schizosaccharomyces pombe
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U2 - 10.1016/j.gene.2014.12.067
DO - 10.1016/j.gene.2014.12.067
M3 - Article
C2 - 25556969
AN - SCOPUS:84922233210
SN - 0378-1119
VL - 558
SP - 241
EP - 253
JO - Gene
JF - Gene
IS - 2
ER -