Glucagon-like peptide 2 enhances intestinal epithelial restitution

Anthony P. Ramsanahie; Alexander Perez; Anette U. Duensing; Michael J. Zinner; Stanley W. Ashley; Edward E. Whang

doi:10.1006/jsre.2002.6504

Glucagon-like peptide 2 enhances intestinal epithelial restitution

Anthony P. Ramsanahie, Alexander Perez, Anette U. Duensing, Michael J. Zinner, Stanley W. Ashley, Edward E. Whang

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Background. Glucagon-like peptide 2 (GLP-2) is a potent intestinotrophic peptide that enhances recovery following intestinal injury. We tested the hypothesis that GLP-2 acutely enhances intestinal epithelial restitution. Material and methods. Rat jejunal segments were mounted in Ussing chambers. HCl (10 mM) was applied to the mucosal surfaces for 10 min to induce injury. Tissues were then lavaged with modified Ringer's solution and maintained in the chambers for an additional 3 h. Tissues were treated with 10 μM GLP-2 or vehicle alone (control). Electrical parameters were recorded, and tissues were salvaged for morphometric analysis. Results. GLP-2-treated tissues exhibited a significantly greater recovery of potential difference and resistance (P < 0.05) than did controls. Morphometric analysis revealed that columnar cells covered a greater percentage of the epithelial surface in GLP-2-treated tissues than in controls (P < 0.05). Conclusions. These results suggest that GLP-2 acutely enhances intestinal epithelial restitution following acid-induced injury. This novel biological action of GLP-2 may contribute to its therapeutic effect in models of intestinal disease.

Original language	English (US)
Pages (from-to)	44-49
Number of pages	6
Journal	Journal of Surgical Research
Volume	107
Issue number	1
DOIs	https://doi.org/10.1006/jsre.2002.6504
State	Published - 2002
Externally published	Yes

Keywords

GLP-2
Restitution
Ussing chamber

ASJC Scopus subject areas

Surgery

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10.1006/jsre.2002.6504

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@article{edbb030592b0467a8edfea01e0ebe854,

title = "Glucagon-like peptide 2 enhances intestinal epithelial restitution",

abstract = "Background. Glucagon-like peptide 2 (GLP-2) is a potent intestinotrophic peptide that enhances recovery following intestinal injury. We tested the hypothesis that GLP-2 acutely enhances intestinal epithelial restitution. Material and methods. Rat jejunal segments were mounted in Ussing chambers. HCl (10 mM) was applied to the mucosal surfaces for 10 min to induce injury. Tissues were then lavaged with modified Ringer's solution and maintained in the chambers for an additional 3 h. Tissues were treated with 10 μM GLP-2 or vehicle alone (control). Electrical parameters were recorded, and tissues were salvaged for morphometric analysis. Results. GLP-2-treated tissues exhibited a significantly greater recovery of potential difference and resistance (P < 0.05) than did controls. Morphometric analysis revealed that columnar cells covered a greater percentage of the epithelial surface in GLP-2-treated tissues than in controls (P < 0.05). Conclusions. These results suggest that GLP-2 acutely enhances intestinal epithelial restitution following acid-induced injury. This novel biological action of GLP-2 may contribute to its therapeutic effect in models of intestinal disease.",

keywords = "GLP-2, Restitution, Ussing chamber",

author = "Ramsanahie, {Anthony P.} and Alexander Perez and Duensing, {Anette U.} and Zinner, {Michael J.} and Ashley, {Stanley W.} and Whang, {Edward E.}",

note = "Funding Information: Presented at the Annual Meeting of the Association for Academic Surgery, Milwaukee, Wisconsin, November 15–17, 2001. 1Supported by National Institutes of Health Grants DK 02786(EEW) and DK 47326(SWA).",

year = "2002",

doi = "10.1006/jsre.2002.6504",

language = "English (US)",

volume = "107",

pages = "44--49",

journal = "Journal of Surgical Research",

issn = "0022-4804",

publisher = "Academic Press Inc.",

number = "1",

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TY - JOUR

T1 - Glucagon-like peptide 2 enhances intestinal epithelial restitution

AU - Ramsanahie, Anthony P.

AU - Perez, Alexander

AU - Duensing, Anette U.

AU - Zinner, Michael J.

AU - Ashley, Stanley W.

AU - Whang, Edward E.

N1 - Funding Information: Presented at the Annual Meeting of the Association for Academic Surgery, Milwaukee, Wisconsin, November 15–17, 2001. 1Supported by National Institutes of Health Grants DK 02786(EEW) and DK 47326(SWA).

PY - 2002

Y1 - 2002

N2 - Background. Glucagon-like peptide 2 (GLP-2) is a potent intestinotrophic peptide that enhances recovery following intestinal injury. We tested the hypothesis that GLP-2 acutely enhances intestinal epithelial restitution. Material and methods. Rat jejunal segments were mounted in Ussing chambers. HCl (10 mM) was applied to the mucosal surfaces for 10 min to induce injury. Tissues were then lavaged with modified Ringer's solution and maintained in the chambers for an additional 3 h. Tissues were treated with 10 μM GLP-2 or vehicle alone (control). Electrical parameters were recorded, and tissues were salvaged for morphometric analysis. Results. GLP-2-treated tissues exhibited a significantly greater recovery of potential difference and resistance (P < 0.05) than did controls. Morphometric analysis revealed that columnar cells covered a greater percentage of the epithelial surface in GLP-2-treated tissues than in controls (P < 0.05). Conclusions. These results suggest that GLP-2 acutely enhances intestinal epithelial restitution following acid-induced injury. This novel biological action of GLP-2 may contribute to its therapeutic effect in models of intestinal disease.

AB - Background. Glucagon-like peptide 2 (GLP-2) is a potent intestinotrophic peptide that enhances recovery following intestinal injury. We tested the hypothesis that GLP-2 acutely enhances intestinal epithelial restitution. Material and methods. Rat jejunal segments were mounted in Ussing chambers. HCl (10 mM) was applied to the mucosal surfaces for 10 min to induce injury. Tissues were then lavaged with modified Ringer's solution and maintained in the chambers for an additional 3 h. Tissues were treated with 10 μM GLP-2 or vehicle alone (control). Electrical parameters were recorded, and tissues were salvaged for morphometric analysis. Results. GLP-2-treated tissues exhibited a significantly greater recovery of potential difference and resistance (P < 0.05) than did controls. Morphometric analysis revealed that columnar cells covered a greater percentage of the epithelial surface in GLP-2-treated tissues than in controls (P < 0.05). Conclusions. These results suggest that GLP-2 acutely enhances intestinal epithelial restitution following acid-induced injury. This novel biological action of GLP-2 may contribute to its therapeutic effect in models of intestinal disease.

KW - GLP-2

KW - Restitution

KW - Ussing chamber

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Glucagon-like peptide 2 enhances intestinal epithelial restitution

Abstract

Keywords

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