Glucagon-like peptide 2 enhances intestinal epithelial restitution

Anthony P. Ramsanahie, Alexander Perez, Anette U. Duensing, Michael J. Zinner, Stanley W. Ashley, Edward E. Whang

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Background. Glucagon-like peptide 2 (GLP-2) is a potent intestinotrophic peptide that enhances recovery following intestinal injury. We tested the hypothesis that GLP-2 acutely enhances intestinal epithelial restitution. Material and methods. Rat jejunal segments were mounted in Ussing chambers. HCl (10 mM) was applied to the mucosal surfaces for 10 min to induce injury. Tissues were then lavaged with modified Ringer's solution and maintained in the chambers for an additional 3 h. Tissues were treated with 10 μM GLP-2 or vehicle alone (control). Electrical parameters were recorded, and tissues were salvaged for morphometric analysis. Results. GLP-2-treated tissues exhibited a significantly greater recovery of potential difference and resistance (P < 0.05) than did controls. Morphometric analysis revealed that columnar cells covered a greater percentage of the epithelial surface in GLP-2-treated tissues than in controls (P < 0.05). Conclusions. These results suggest that GLP-2 acutely enhances intestinal epithelial restitution following acid-induced injury. This novel biological action of GLP-2 may contribute to its therapeutic effect in models of intestinal disease.

Original languageEnglish (US)
Pages (from-to)44-49
Number of pages6
JournalJournal of Surgical Research
Volume107
Issue number1
StatePublished - 2002
Externally publishedYes

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Keywords

  • GLP-2
  • Restitution
  • Ussing chamber

ASJC Scopus subject areas

  • Surgery

Cite this

Ramsanahie, A. P., Perez, A., Duensing, A. U., Zinner, M. J., Ashley, S. W., & Whang, E. E. (2002). Glucagon-like peptide 2 enhances intestinal epithelial restitution. Journal of Surgical Research, 107(1), 44-49.