Glucan phosphate treatment attenuates burn-induced inflammation and improves resistance to Pseudomonas aeruginosa burn wound infection

Olga I. Lyuksutova, Erle D. Murphey, Tracy Toliver-Kinsky, Cheng Y. Lin, Weihua Cui, David L. Williams, Edward R. Sherwood

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

These studies evaluated the effects treatment with glucan phosphate, a soluble polysaccharide immunomodulator, on the inflammatory response induced by burn injury and on resistance to Pseudomonas aeruginosa burn wound infection. Mice were exposed to 35% total body surface area burns and were resuscitated with lactated Ringer's (LR) solution alone or LR supplemented with glucan phosphate (40 mg/kg). Glucan phosphate treatment attenuated burn-induced expression of interleukin (IL)-1β, IL-6, and IL-10 mRNAs in spleen, lung, and heart. Plasma concentrations of IL-1β, IL-6, macrophage inflammatory protein (MIP)-2, and IL-10 were also decreased in burned mice treated with glucan phosphate compared with vehicle-treated controls. Early postburn mortality was not significantly different between control (20%) and glucan phosphate-treated (10%) mice, but there was a small improvement in acid-base balance in the glucan phosphate-treated group. Mice received a second injection of glucan phosphate or LR on day 4 postburn and were infected by topical application of P. aeruginosa to the burn wound on day 5. Glucan phosphate treatment significantly improved survival in mice exposed to P. aeruginosa burn wound infection. The improved survival correlated with lower bacterial burden in the burn wound, attenuated production of proinflammatory cytokines, and enhanced production of Th1 cytokines. These studies show that glucan phosphate treatment attenuates burn-induced inflammation and increases resistance to P. aeruginosa burn wound infection in an experimental model of burn injury.

Original languageEnglish (US)
Pages (from-to)224-232
Number of pages9
JournalShock
Volume23
Issue number3
DOIs
StatePublished - Mar 2005

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Wound Infection
Burns
Pseudomonas aeruginosa
Inflammation
Therapeutics
Wounds and Injuries
Interleukin-1
Interleukin-10
Interleukin-6
Chemokine CXCL2
Cytokines
glucan phosphate
Acid-Base Equilibrium
Body Surface Area
Immunologic Factors
Polysaccharides
Theoretical Models
Spleen
Lung
Messenger RNA

Keywords

  • Cytokines
  • Immunomodulation
  • Infection
  • Shock
  • Trauma

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Physiology

Cite this

Glucan phosphate treatment attenuates burn-induced inflammation and improves resistance to Pseudomonas aeruginosa burn wound infection. / Lyuksutova, Olga I.; Murphey, Erle D.; Toliver-Kinsky, Tracy; Lin, Cheng Y.; Cui, Weihua; Williams, David L.; Sherwood, Edward R.

In: Shock, Vol. 23, No. 3, 03.2005, p. 224-232.

Research output: Contribution to journalArticle

Lyuksutova, Olga I. ; Murphey, Erle D. ; Toliver-Kinsky, Tracy ; Lin, Cheng Y. ; Cui, Weihua ; Williams, David L. ; Sherwood, Edward R. / Glucan phosphate treatment attenuates burn-induced inflammation and improves resistance to Pseudomonas aeruginosa burn wound infection. In: Shock. 2005 ; Vol. 23, No. 3. pp. 224-232.
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