Glucocorticoid modulation of β-adrenergic receptors of cultured rat arterial smooth muscle cells

A. Jazayeri, Walter Meyer

Research output: Contribution to journalArticle

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Abstract

Since both glucocorticoids and catecholamines are involved in the regulation of normal blood pressure, we investigated the modulation of β-adrenergic receptors of cultured rat arterial smooth muscle cells by glucocorticoids. The synthetic glucocorticoids dexamethasone and RU 28362, at 10-8 M concentration, increased maximum β-adrenergic binding but had no effect on the dissociation constant (K(d)). Each steroid caused an increase in maximum [3H]dihydroalprenonol binding over the concentration range of 10-8 to 10-6 M, but not at 10-9 M. The glucocorticoid effect on β-adrenergic receptors of arterial smooth muscle cells required a minimum of 20 hours of incubation in the presence of the steroid and was significantly inhibited by cycloheximide (10 μg/ml), indicating that the glucocorticoid effect required protein synthesis. The effect of dexamethasone on [3H]dihydroalprenolol binding was significantly inhibited by the glucocorticoid antagonist RU 38486. Basal and agonist-stimulated cyclic adenosine 3',5'-monophosphate (cAMP) levels in arterial smooth muscle cells, before and after glucocorticoid treatment, were measured as an indicator of the physiological significance of the observed glucocorticoid-induced increase in β-adrenergic receptor binding. While causing no change in the basal cAMP level, treatment of arterial smooth muscle cells with 10-6 M dexamethasone for 24 hours increased the 10-6 M isoproterenol-stimulated cAMP levels.

Original languageEnglish (US)
Pages (from-to)393-398
Number of pages6
JournalHypertension
Volume12
Issue number4
StatePublished - 1988

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Adrenergic Receptors
Glucocorticoids
Smooth Muscle Myocytes
Cyclic AMP
Dexamethasone
Steroids
Dihydroalprenolol
Mifepristone
Cycloheximide
Isoproterenol
Adrenergic Agents
Catecholamines
Blood Pressure
Proteins

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Glucocorticoid modulation of β-adrenergic receptors of cultured rat arterial smooth muscle cells. / Jazayeri, A.; Meyer, Walter.

In: Hypertension, Vol. 12, No. 4, 1988, p. 393-398.

Research output: Contribution to journalArticle

Jazayeri, A. ; Meyer, Walter. / Glucocorticoid modulation of β-adrenergic receptors of cultured rat arterial smooth muscle cells. In: Hypertension. 1988 ; Vol. 12, No. 4. pp. 393-398.
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