Glucocorticoid receptor characteristics in healthy childrens' leukocytes: Correlation with gender, weight, age and plasma cortisol

J. Aljubeh, A. Miller, Walter Meyer, B. Thompson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This study evaluated the glucocorticoid receptor (GR) binding capacity of mononuclear leukocytes (MNL) in male and female, obese and non-obese children and adolescents. Fifteen male and eleven female subjects, ages 6.3 to 21 years, approximately equally divided between obese and non-obese were included. The age distribution was equivalent among groups. Average GR binding capacity and apparent dissociation constant (Kd) did not differ significantly between the obese and non-obese groups. However, females averaged 7280 ± 1440 GR sites/MNL, significantly more than males with 5720 ± 1132 sites/MNL (p = 0.005). There was no significant difference in average Kd between the groups nor was there a difference in the GR characteristics with respect to subjects' age, plasma cortisol concentrations, or degree of adiposity. While in the group studied, there were no differences in MNL GR characteristics based on subjects' weight, age or plasma cortisol, female subjects' MNL averaged 27% greater GR binding capacity than males. This difference appears to be concentrated in the pubertal subgroup.

Original languageEnglish (US)
Pages (from-to)1641-1647
Number of pages7
JournalJournal of Pediatric Endocrinology and Metabolism
Volume14
Issue number9
StatePublished - 2001

Fingerprint

Glucocorticoid Receptors
Mononuclear Leukocytes
Hydrocortisone
Leukocytes
Weights and Measures
Age Distribution
Adiposity

Keywords

  • Child
  • Cortisol
  • Gender
  • Glucocorticoid
  • Glucocorticoid receptor
  • Leukocyte
  • Obesity

ASJC Scopus subject areas

  • Endocrinology
  • Pediatrics, Perinatology, and Child Health

Cite this

Glucocorticoid receptor characteristics in healthy childrens' leukocytes : Correlation with gender, weight, age and plasma cortisol. / Aljubeh, J.; Miller, A.; Meyer, Walter; Thompson, B.

In: Journal of Pediatric Endocrinology and Metabolism, Vol. 14, No. 9, 2001, p. 1641-1647.

Research output: Contribution to journalArticle

@article{c659234af7ef420e9e6af6a3b99a44fc,
title = "Glucocorticoid receptor characteristics in healthy childrens' leukocytes: Correlation with gender, weight, age and plasma cortisol",
abstract = "This study evaluated the glucocorticoid receptor (GR) binding capacity of mononuclear leukocytes (MNL) in male and female, obese and non-obese children and adolescents. Fifteen male and eleven female subjects, ages 6.3 to 21 years, approximately equally divided between obese and non-obese were included. The age distribution was equivalent among groups. Average GR binding capacity and apparent dissociation constant (Kd) did not differ significantly between the obese and non-obese groups. However, females averaged 7280 ± 1440 GR sites/MNL, significantly more than males with 5720 ± 1132 sites/MNL (p = 0.005). There was no significant difference in average Kd between the groups nor was there a difference in the GR characteristics with respect to subjects' age, plasma cortisol concentrations, or degree of adiposity. While in the group studied, there were no differences in MNL GR characteristics based on subjects' weight, age or plasma cortisol, female subjects' MNL averaged 27{\%} greater GR binding capacity than males. This difference appears to be concentrated in the pubertal subgroup.",
keywords = "Child, Cortisol, Gender, Glucocorticoid, Glucocorticoid receptor, Leukocyte, Obesity",
author = "J. Aljubeh and A. Miller and Walter Meyer and B. Thompson",
year = "2001",
language = "English (US)",
volume = "14",
pages = "1641--1647",
journal = "Journal of Pediatric Endocrinology and Metabolism",
issn = "0334-018X",
publisher = "Walter de Gruyter GmbH & Co. KG",
number = "9",

}

TY - JOUR

T1 - Glucocorticoid receptor characteristics in healthy childrens' leukocytes

T2 - Correlation with gender, weight, age and plasma cortisol

AU - Aljubeh, J.

AU - Miller, A.

AU - Meyer, Walter

AU - Thompson, B.

PY - 2001

Y1 - 2001

N2 - This study evaluated the glucocorticoid receptor (GR) binding capacity of mononuclear leukocytes (MNL) in male and female, obese and non-obese children and adolescents. Fifteen male and eleven female subjects, ages 6.3 to 21 years, approximately equally divided between obese and non-obese were included. The age distribution was equivalent among groups. Average GR binding capacity and apparent dissociation constant (Kd) did not differ significantly between the obese and non-obese groups. However, females averaged 7280 ± 1440 GR sites/MNL, significantly more than males with 5720 ± 1132 sites/MNL (p = 0.005). There was no significant difference in average Kd between the groups nor was there a difference in the GR characteristics with respect to subjects' age, plasma cortisol concentrations, or degree of adiposity. While in the group studied, there were no differences in MNL GR characteristics based on subjects' weight, age or plasma cortisol, female subjects' MNL averaged 27% greater GR binding capacity than males. This difference appears to be concentrated in the pubertal subgroup.

AB - This study evaluated the glucocorticoid receptor (GR) binding capacity of mononuclear leukocytes (MNL) in male and female, obese and non-obese children and adolescents. Fifteen male and eleven female subjects, ages 6.3 to 21 years, approximately equally divided between obese and non-obese were included. The age distribution was equivalent among groups. Average GR binding capacity and apparent dissociation constant (Kd) did not differ significantly between the obese and non-obese groups. However, females averaged 7280 ± 1440 GR sites/MNL, significantly more than males with 5720 ± 1132 sites/MNL (p = 0.005). There was no significant difference in average Kd between the groups nor was there a difference in the GR characteristics with respect to subjects' age, plasma cortisol concentrations, or degree of adiposity. While in the group studied, there were no differences in MNL GR characteristics based on subjects' weight, age or plasma cortisol, female subjects' MNL averaged 27% greater GR binding capacity than males. This difference appears to be concentrated in the pubertal subgroup.

KW - Child

KW - Cortisol

KW - Gender

KW - Glucocorticoid

KW - Glucocorticoid receptor

KW - Leukocyte

KW - Obesity

UR - http://www.scopus.com/inward/record.url?scp=0035677505&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035677505&partnerID=8YFLogxK

M3 - Article

C2 - 11795655

AN - SCOPUS:0035677505

VL - 14

SP - 1641

EP - 1647

JO - Journal of Pediatric Endocrinology and Metabolism

JF - Journal of Pediatric Endocrinology and Metabolism

SN - 0334-018X

IS - 9

ER -