GLUT-3 expression in human skeletal muscle

Charles A. Stuart, Gary Wen, Bihung Peng, Vsevolod Popov, S. David Hudnall, Gerald Campbell

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Muscle biopsy homogenates contain GLUT-3 mRNA and protein. Before these studies, it was unclear where GLUT-3 was located in muscle tissue. In situ hybridization using a mid-molecule probe demonstrated GLUT-3 within all muscle fibers. Fluorescent-tagged antibody reacting with affinity-purified antibody directed at the carboxy-terminus demonstrated GLUT-3 protein in all fibers. Slow-twitch muscle fibers, identified by NADH-tetrazolium reductase staining, possessed more GLUT-3 protein than fast-twitch fibers. Electron microscopy using affinity-purified primary antibody and gold particle-tagged second antibody showed that the majority of GLUT-3 was in association with triads and transverse tubules inside the fiber. Strong GLUT-3 signals were seen in association with the few nerves that traversed muscle sections. Electron microscopic evaluation of human peripheral nerve demonstrated GLUT-3 within the axon, with many of the particles related to mitochondria. GLUT-3 protein was found in myelin but not in Schwann cells. GLUT-1 protein was not present in nerve cells, axons, myelin, or Schwann cells but was seen at the surface of the peripheral nerve in the perineurium. These studies demonstrated that GLUT-3 mRNA and protein are expressed throughout normal human skeletal muscle, but the protein is predominantly found in the triads of slow-twitch muscle fibers.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume279
Issue number4 42-4
StatePublished - 2000

Fingerprint

Glucose Transporter Type 3
Muscle
Skeletal Muscle
Peripheral Nerves
Fibers
Slow-Twitch Muscle Fibers
Muscles
Schwann Cells
Myelin Sheath
Antibodies
Axons
NADH Tetrazolium Reductase
Glucose Transporter Type 1
Messenger RNA
Cells
Antibody Affinity
Muscle Proteins
Mitochondria
Biopsy
Gold

Keywords

  • GLUT-3
  • Human muscle
  • Immunofluorescence
  • In situ hybridization

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry
  • Physiology (medical)

Cite this

GLUT-3 expression in human skeletal muscle. / Stuart, Charles A.; Wen, Gary; Peng, Bihung; Popov, Vsevolod; Hudnall, S. David; Campbell, Gerald.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 279, No. 4 42-4, 2000.

Research output: Contribution to journalArticle

Stuart, Charles A. ; Wen, Gary ; Peng, Bihung ; Popov, Vsevolod ; Hudnall, S. David ; Campbell, Gerald. / GLUT-3 expression in human skeletal muscle. In: American Journal of Physiology - Endocrinology and Metabolism. 2000 ; Vol. 279, No. 4 42-4.
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