Glutamate decarboxylase

Jang Yen Wu, Larry A. Denner, Chin Tarng Lin, Bang Hwang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

L-Glutamate decarboxylase (GAD, EC4.1.1.15) which catalyzes the conversion of Lglutamate to γ-aminobutyric acid (GABA), an important inhibitory neurotransmitter, is a specific marker for GABAergic neurons and their processes.1-4 Although GABA and GAD were originally believed to exist exclusively in the central nervous system (CNS) of the vertebrate5, with more sensitive methods GABA and GAD activity has been detected in glia and nonneural tissues such as the kidney, heart, liver, pancreas, adrenal medulla, and blood vessels.6-16 However, little is known with certainty about the properties of glial or nonneural GAD. Contrary to the glial GAD, the neuronal GAD has been purified to homogeneity from several species including the mouse,17 rat,18,19 bovine,20 catfish,21 and human22 and its properties have also been extensively characterized.23-27In a ddition, specific polyclonal and monoclonal antibodies against the neuronal GAD have also been obtained and characterized'28-31 and applied extensively for immunochemical and immunocytochemical studies of GAD in the vertebrate (for a review see References 2 to 4 and 32). In this review, the authors would like to cover the purification procedures, the criteria of purity, and the basic kinetic, physical, and immunochemical properties of GAD in addition to their application of the identification of GABAergic neurons and their projections.

Original languageEnglish (US)
Title of host publicationGlutamine and Glutamate Mammals
Subtitle of host publicationVolume I
PublisherCRC Press
Pages101-122
Number of pages22
ISBN (Electronic)9781351080743
ISBN (Print)9781315893198
DOIs
StatePublished - Jan 1 2018
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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  • Cite this

    Wu, J. Y., Denner, L. A., Lin, C. T., & Hwang, B. (2018). Glutamate decarboxylase. In Glutamine and Glutamate Mammals: Volume I (pp. 101-122). CRC Press. https://doi.org/10.1201/9781351072298