Glutamate immunoreactivity in rat dorsal root axons

Karin N. Westlund; Daniel L. McNeill; Richard E. Coggeshall

doi:10.1016/0304-3940(89)90235-8

Glutamate immunoreactivity in rat dorsal root axons

Karin N. Westlund, Daniel L. McNeill, Richard E. Coggeshall

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93 Scopus citations

Abstract

Approximately 8.5% of the unmyelinated and 2.5% of the myelinated primary afferent axons in lumbar dorsal roots of normal rats are immunostained for glutamate. Thus unmyelinated fibers are the predominantly immunostained population under the conditions of our experiments. The mean size of the unmyelinated fibers is greater at L₆ than at L₄ and L₂. The meaning of this is not clear, but it may imply that pelvic visceral afferents are slightly larger than afferents from other areas. We emphasize that the immunostained axons can be demonstrated in otherwise normal animals, so changes in the percentages of labeled axons in response to various stimuli will be of interest.

Original language	English (US)
Pages (from-to)	13-17
Number of pages	5
Journal	Neuroscience Letters
Volume	96
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(89)90235-8
State	Published - Jan 2 1989

Keywords

Glutamate
Immunocytochemistry
Primary afferent fiber

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(89)90235-8

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@article{df928f7678594889976e9919cf72665f,

title = "Glutamate immunoreactivity in rat dorsal root axons",

abstract = "Approximately 8.5% of the unmyelinated and 2.5% of the myelinated primary afferent axons in lumbar dorsal roots of normal rats are immunostained for glutamate. Thus unmyelinated fibers are the predominantly immunostained population under the conditions of our experiments. The mean size of the unmyelinated fibers is greater at L6 than at L4 and L2. The meaning of this is not clear, but it may imply that pelvic visceral afferents are slightly larger than afferents from other areas. We emphasize that the immunostained axons can be demonstrated in otherwise normal animals, so changes in the percentages of labeled axons in response to various stimuli will be of interest.",

keywords = "Glutamate, Immunocytochemistry, Primary afferent fiber",

author = "Westlund, {Karin N.} and McNeill, {Daniel L.} and Coggeshall, {Richard E.}",

note = "Funding Information: This work is partiallys upportedby NIH GrantsN S11255a ndN S10161b, y a fellowship from the Kent WaldrepN ationalP aralysisF oundationb,y the Texas Affi-liateo f the AmericaHne artA ssociationb,y the Florencaen dMarie Hall Endowment for Excellencien Medical Educationa, ndby an awardfr omthe Bristol-MeyerCso m-pany. The authorsg ratefullya cknowledgteh e assistancoef Ms. Judy Pettett,M s. TamaraS herburna nd Ms. LavernW ilson.We are also gratefutl o Drs. Alan Beitz and AldoR ustionif or donatingth eantibodies.",

year = "1989",

month = jan,

day = "2",

doi = "10.1016/0304-3940(89)90235-8",

language = "English (US)",

volume = "96",

pages = "13--17",

journal = "Neuroscience Letters",

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number = "1",

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TY - JOUR

T1 - Glutamate immunoreactivity in rat dorsal root axons

AU - Westlund, Karin N.

AU - McNeill, Daniel L.

AU - Coggeshall, Richard E.

N1 - Funding Information: This work is partiallys upportedby NIH GrantsN S11255a ndN S10161b, y a fellowship from the Kent WaldrepN ationalP aralysisF oundationb,y the Texas Affi-liateo f the AmericaHne artA ssociationb,y the Florencaen dMarie Hall Endowment for Excellencien Medical Educationa, ndby an awardfr omthe Bristol-MeyerCso m-pany. The authorsg ratefullya cknowledgteh e assistancoef Ms. Judy Pettett,M s. TamaraS herburna nd Ms. LavernW ilson.We are also gratefutl o Drs. Alan Beitz and AldoR ustionif or donatingth eantibodies.

PY - 1989/1/2

Y1 - 1989/1/2

N2 - Approximately 8.5% of the unmyelinated and 2.5% of the myelinated primary afferent axons in lumbar dorsal roots of normal rats are immunostained for glutamate. Thus unmyelinated fibers are the predominantly immunostained population under the conditions of our experiments. The mean size of the unmyelinated fibers is greater at L6 than at L4 and L2. The meaning of this is not clear, but it may imply that pelvic visceral afferents are slightly larger than afferents from other areas. We emphasize that the immunostained axons can be demonstrated in otherwise normal animals, so changes in the percentages of labeled axons in response to various stimuli will be of interest.

AB - Approximately 8.5% of the unmyelinated and 2.5% of the myelinated primary afferent axons in lumbar dorsal roots of normal rats are immunostained for glutamate. Thus unmyelinated fibers are the predominantly immunostained population under the conditions of our experiments. The mean size of the unmyelinated fibers is greater at L6 than at L4 and L2. The meaning of this is not clear, but it may imply that pelvic visceral afferents are slightly larger than afferents from other areas. We emphasize that the immunostained axons can be demonstrated in otherwise normal animals, so changes in the percentages of labeled axons in response to various stimuli will be of interest.

KW - Glutamate

KW - Immunocytochemistry

KW - Primary afferent fiber

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AN - SCOPUS:0024581612

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VL - 96

SP - 13

EP - 17

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1

ER -

Glutamate immunoreactivity in rat dorsal root axons

Abstract

Keywords

ASJC Scopus subject areas

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