Glutamatergic and GABAergic input to rat spinothalamic tract cells in the superficial dorsal horn

H. A. Lekan, S. M. Carlton

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    The distribution of synaptic terminals onto spinothalamic tract cells (types I and II) of the superficial dorsal horn was determined with special reference to the amino acid transmitters glutamate and γ-aminobutyric acid. Fifteen spinothalamic cells retrogradely labeled from the thalamus with the neuroanatomical tracer wheatgerm agglutinin conjugated to horseradish peroxidase were sectioned for electron microscopy. Serial sections from several levels through each cell were immunostained for glutamate and γ- aminobutyric acid using a postembedding immunogold technique. Perimeter measurements of spinothalamic cell somata and dendrites and the lengths of apposition for all terminal profiles in contact with the spinothalamic cells were obtained from electron micrographs using a digitizing tablet. These data were used to determine the density of terminals on the soma and dendrites. In addition, the terminal population on these cells was categorized by transmitter content (glutamate, γ-aminobutyric acid, or unlabeled). The results demonstrate that terminal density increased on dendrites relative to their distance from the soma. Glutamatergic and GABAergic input composed 37% and 20% of the terminal population, respectively, and these percentages remained uniform for the soma and dendrites. There were no significant differences among the 15 cells analyzed for this study. The results, therefore, suggest that both type I and type II STT cells of the superficial DH have similar synaptic organizations.

    Original languageEnglish (US)
    Pages (from-to)417-428
    Number of pages12
    JournalJournal of Comparative Neurology
    Volume361
    Issue number3
    DOIs
    StatePublished - 1995

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    Spinothalamic Tracts
    Carisoprodol
    Dendrites
    Aminobutyrates
    Glutamic Acid
    Spinal Cord Dorsal Horn
    Agglutinins
    Presynaptic Terminals
    Horseradish Peroxidase
    Thalamus
    Population
    Tablets
    Electron Microscopy
    Immunohistochemistry
    Electrons
    Amino Acids

    Keywords

    • ascending systems
    • immunogold
    • lamina I
    • nociception

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Glutamatergic and GABAergic input to rat spinothalamic tract cells in the superficial dorsal horn. / Lekan, H. A.; Carlton, S. M.

    In: Journal of Comparative Neurology, Vol. 361, No. 3, 1995, p. 417-428.

    Research output: Contribution to journalArticle

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