Glutathione depletion in rats impairs T-cell and macrophage immune function

M. K. Robinson, M. L. Rodrick, D. O. Jacobs, J. D. Rounds, K. H. Collins, I. B. Saporoschetz, J. A. Mannick, D. W. Wilmore

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Critical illness is associated with both immunosuppression and glutathione deficiency. We determined if in vivo depletion of glutathione would adversely affect immune status. Rats with normal glutathione levels and those with glutathione stores depleted by diethyl maleate underwent analysis of splenocyte function and mesenteric lymph node lymphocyte function. Lymphocytes of the spleen and mesenteric lymph nodes were tested for concanavalin A proliferative response and interleukin 2 production. Tumor necrosis factor and interleukin 6 secretion by splenic adherent cells was also measured. Glutathione-depleted animals had significantly decreased lymphocyte proliferation and decreased production of tumor necrosis factor and interleukin 6 but unaltered interleukin 2 production. These findings indicate that in vivo glutathione deficiency impairs macrophage and T-cell function. Because glutathione depletion may occur in sepsis, trauma, and shock, treatments that help maintain glutathione levels may enhance immunocompetence and thus improve the ability of patients to recover from critical illness.

Original languageEnglish (US)
Pages (from-to)29-35
Number of pages7
JournalArchives of Surgery
Volume128
Issue number1
StatePublished - 1993
Externally publishedYes

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Glutathione
Macrophages
T-Lymphocytes
diethyl maleate
Lymphocytes
Critical Illness
Interleukin-2
Interleukin-6
Tumor Necrosis Factor-alpha
Lymph Nodes
Immunocompetence
Concanavalin A
Immunosuppression
Shock
Sepsis
Spleen
Wounds and Injuries

ASJC Scopus subject areas

  • Surgery

Cite this

Robinson, M. K., Rodrick, M. L., Jacobs, D. O., Rounds, J. D., Collins, K. H., Saporoschetz, I. B., ... Wilmore, D. W. (1993). Glutathione depletion in rats impairs T-cell and macrophage immune function. Archives of Surgery, 128(1), 29-35.

Glutathione depletion in rats impairs T-cell and macrophage immune function. / Robinson, M. K.; Rodrick, M. L.; Jacobs, D. O.; Rounds, J. D.; Collins, K. H.; Saporoschetz, I. B.; Mannick, J. A.; Wilmore, D. W.

In: Archives of Surgery, Vol. 128, No. 1, 1993, p. 29-35.

Research output: Contribution to journalArticle

Robinson, MK, Rodrick, ML, Jacobs, DO, Rounds, JD, Collins, KH, Saporoschetz, IB, Mannick, JA & Wilmore, DW 1993, 'Glutathione depletion in rats impairs T-cell and macrophage immune function', Archives of Surgery, vol. 128, no. 1, pp. 29-35.
Robinson MK, Rodrick ML, Jacobs DO, Rounds JD, Collins KH, Saporoschetz IB et al. Glutathione depletion in rats impairs T-cell and macrophage immune function. Archives of Surgery. 1993;128(1):29-35.
Robinson, M. K. ; Rodrick, M. L. ; Jacobs, D. O. ; Rounds, J. D. ; Collins, K. H. ; Saporoschetz, I. B. ; Mannick, J. A. ; Wilmore, D. W. / Glutathione depletion in rats impairs T-cell and macrophage immune function. In: Archives of Surgery. 1993 ; Vol. 128, No. 1. pp. 29-35.
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