Glutathione-linked pathways in drug resistance: Characterization and functional reconstitution of human erythrocyte glutathione-conjugate transporter

Y. C. Awasthi, S. S. Singhal, P. Zimniak, J. T. Piper, S. Pikula, J. Bandorowicz-Pikula, J. T. Lin, Satish Srivastava, S. V. Singh, S. Awasthi

Research output: Contribution to journalArticle

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Abstract

The glutathione-conjugate transporter, S-(2,4-Dinitrophenyl) glutathione (DNP-SG) ATPase described by us previously has been purified from human erythrocytes. DNP-SG ATPase showed a band at 38 kDa in SDS gels which was not recognized by the monoclonal antibodies against multidrug resistance associated protein (MRP). A saturable photoaffinity labeling of the 38 kDa band was observed with 8-azido ATP (K(d) = 2 μM). The transporter catalyzed ATP hydrolysis which was stimulated in the presence of glutathione-conjugate of 1-chloro-2,4-dinitro benzene (DNP-SG) as well as the cationic amphiphilic chemotherapeutic drug, doxorubicin (DOX). When reconstituted in artificial liposomes, DNP-SG ATPase mediated ATP-dependent, saturable transport of DOX (K(m) 2.4 μM, V(max) 194 nmol/min/mg) as well as DNP-SG (K(m) 36 μM, V(max) 433 nmol/min/mg). The K(m) for ATP for both substrates was about 2.5 mM. Transport of DOX was competitively inhibited by DNP-SG and vice versa.

Original languageEnglish (US)
Pages (from-to)431-448
Number of pages18
JournalClinical Chemistry and Enzymology Communications
Volume8
Issue number4-6
StatePublished - 1999

Fingerprint

Drug Resistance
Doxorubicin
Glutathione
Adenosine Triphosphatases
Adenosine Triphosphate
Erythrocytes
Pharmaceutical Preparations
Multidrug Resistance-Associated Proteins
Dinitrochlorobenzene
Liposomes
Labeling
Hydrolysis
Gels
Monoclonal Antibodies
Substrates
glutathione transporter

Keywords

  • Doxorubicin
  • Drug resistance
  • Glutathione
  • Xenobiotic transport

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Awasthi, Y. C., Singhal, S. S., Zimniak, P., Piper, J. T., Pikula, S., Bandorowicz-Pikula, J., ... Awasthi, S. (1999). Glutathione-linked pathways in drug resistance: Characterization and functional reconstitution of human erythrocyte glutathione-conjugate transporter. Clinical Chemistry and Enzymology Communications, 8(4-6), 431-448.

Glutathione-linked pathways in drug resistance : Characterization and functional reconstitution of human erythrocyte glutathione-conjugate transporter. / Awasthi, Y. C.; Singhal, S. S.; Zimniak, P.; Piper, J. T.; Pikula, S.; Bandorowicz-Pikula, J.; Lin, J. T.; Srivastava, Satish; Singh, S. V.; Awasthi, S.

In: Clinical Chemistry and Enzymology Communications, Vol. 8, No. 4-6, 1999, p. 431-448.

Research output: Contribution to journalArticle

Awasthi, YC, Singhal, SS, Zimniak, P, Piper, JT, Pikula, S, Bandorowicz-Pikula, J, Lin, JT, Srivastava, S, Singh, SV & Awasthi, S 1999, 'Glutathione-linked pathways in drug resistance: Characterization and functional reconstitution of human erythrocyte glutathione-conjugate transporter', Clinical Chemistry and Enzymology Communications, vol. 8, no. 4-6, pp. 431-448.
Awasthi, Y. C. ; Singhal, S. S. ; Zimniak, P. ; Piper, J. T. ; Pikula, S. ; Bandorowicz-Pikula, J. ; Lin, J. T. ; Srivastava, Satish ; Singh, S. V. ; Awasthi, S. / Glutathione-linked pathways in drug resistance : Characterization and functional reconstitution of human erythrocyte glutathione-conjugate transporter. In: Clinical Chemistry and Enzymology Communications. 1999 ; Vol. 8, No. 4-6. pp. 431-448.
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