Glutathione S-transferase-catalyzed conjugation of 9,10-epoxystearic acid with glutathione.

R. Sharma, S. Gupta, S. S. Singhal, Ghulam Ansari, Y. C. Awasthi

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Abstract

The possible role of glutathione S-transferases (GST) in detoxification of fatty acid epoxides generated during lipid peroxidation has been evaluated. Present studies showed that cytosolic human glutathione S-transferases belonging to alpha, mu, and pi classes isolated from human liver and lung catalyzed the conjugation of glutathione and 9,10-epoxystearic acid. The product of enzymatic reaction, i.e., conjugate of GSH and epoxystearic acid, was isolated and characterized. The Michaelis constant (Km) values of the alpha, mu, and pi classes of GSTs for 9,10-epoxystearic acid were found to be 0.47, 0.32 and 0.80 mM, respectively, whereas the maximal velocity (V max) values for the alpha, mu, and pi classes of GSTs were found to be 142, 256, and 52 mol/min/mol, respectively. These results indicate that even though 9,10-epoxystearic acid is a substrate for all the three classes of GSTs, the mu class isozymes have maximum activity toward this substrate and may preferentially metabolize fatty acid epoxides more effectively as compared to the other classes of GSTs.

Original languageEnglish (US)
Pages (from-to)147-153
Number of pages7
JournalJournal of Biochemical Toxicology
Volume6
Issue number2
StatePublished - Jun 1991

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Glutathione Transferase
Glutathione
Epoxy Compounds
Fatty Acids
Detoxification
Substrates
Liver
Lipid Peroxidation
Isoenzymes
Lipids
Lung
Acids
9,10-epoxystearic acid

ASJC Scopus subject areas

  • Medicine(all)

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Glutathione S-transferase-catalyzed conjugation of 9,10-epoxystearic acid with glutathione. / Sharma, R.; Gupta, S.; Singhal, S. S.; Ansari, Ghulam; Awasthi, Y. C.

In: Journal of Biochemical Toxicology, Vol. 6, No. 2, 06.1991, p. 147-153.

Research output: Contribution to journalArticle

Sharma, R. ; Gupta, S. ; Singhal, S. S. ; Ansari, Ghulam ; Awasthi, Y. C. / Glutathione S-transferase-catalyzed conjugation of 9,10-epoxystearic acid with glutathione. In: Journal of Biochemical Toxicology. 1991 ; Vol. 6, No. 2. pp. 147-153.
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